First Use of the Biocoupler Temporary Immersion System for Micropropagation of Argania spinosa (L.) Skeels : A Step Toward Sustainable Plant Biotechnology

TESSE, Rajaa; BAHLAOUAN, Bouchaib; MRABET, Oumaima; El ANTRI, Said; BOUTALEB, Nadia

doi:10.22059/ijhst.2025.391253.1039

First Use of the Biocoupler Temporary Immersion System for Micropropagation of Argania spinosa (L.) Skeels : A Step Toward Sustainable Plant Biotechnology

Document Type : Research paper

Authors

Rajaa TESSE ¹

Bouchaib BAHLAOUAN ²

Oumaima MRABET ¹

Said El ANTRI ³

Nadia BOUTALEB ³

¹ Hassan II University of Casablanca, Laboratory of Biochemistry, Environment and Agri-Food, LBEA URAC36, 20650 Morocco.

² Higher Institutes of the Nursing Professions and Techniques of Health ISPITS Casablanca 22500, Morocco.

³ Hassan 2 University of Casablanca, Laboratory of Biochemistry, Environment and Agri-Food, LBEA URAC36, 20650 Morocco.

10.22059/ijhst.2025.391253.1039

Abstract

This research introduces the Biocoupler temporary immersion system as a novel method for the micropropagation of Argania spinosa, a species of significant horticultural and ecological value. The primary goal was to refine in vitro propagation conditions to promote robust plant growth while maintaining sustainability. A one-minute immersion cycle proved optimal for stimulating shoot development. By carefully adjusting immersion intervals at three critical growth stages, issues related to waterlogging and vitrification were effectively mitigated. Furthermore, supplementing the culture medium with 0.8 mg L-1 meta-topolin in combination with a low concentration of IBA markedly increased shoot biomass, highlighting a synergistic interaction in growth regulation. Compared to traditional micropropagation techniques, the Biocoupler system provided precise control over moisture and nutrient delivery, substantially lowering the incidence of hyperhydricity. These findings represent a notable advancement in sustainable plant biotechnology, offering a scalable and efficient strategy for both the conservation and commercial propagation of argan trees.

Keywords

Argania Spinosa

Biocoupler

Hyperhydricity

Meta-topolin