International Journal of Horticultural Science and Technology

International Journal of Horticultural Science and Technology

Evaluation of the Nematicidal Activity of Millettia pachyloba Drake Aqueous Extract Against Meloidogyne incognita in Cavendish Banana

Document Type : Research paper

Authors
Bui Hong Quan
Tran Thi Phuong Nhung
Institute of Biotechnology and Food Technology, Industrial University of Ho Chi Minh City, Ho Chi Minh City, Vietnam
10.22059/ijhst.2025.387055.986
Abstract
Root-knot nematodes (Meloidogyne incognita) threaten banana production, and chemical controls pose environmental risks. Millettia pachyloba Drake contains bioactive compounds that can manage pests sustainably. This study investigates the nematicidal effects of M. pachyloba aqueous extract (EAMP) on M. incognita in Cavendish banana plants. Various EAMP concentrations (50, 100, 150, and 200 ppm) were tested for their effects on egg hatching, nematode mortality, enzyme activity, and plant health. The water control group showed no inhibition of egg hatching (0.00 ± 0.00%) (p < 0.05), while Oxamyl significantly reduced hatching to 13.11 ± 0.27% (p < 0.05). EAMP demonstrated a concentration-dependent reduction in egg hatching, with 200 ppm (EAMP200) showing the highest efficacy (23.13 ± 0.23%) (p < 0.05). Nematode mortality increased with concentration, with EAMP200 achieving a mortality rate of 71.74 ± 7.34% at 72 hours (p < 0.05). EAMP also reduced the root-knot index (RKI) and egg mass in a dose-dependent manner, with EAMP500 reducing RKI to 1.33 ± 0.58 and egg mass to 82.67 ± 2.08 (p < 0.05). Furthermore, EAMP treatments improved chlorophyll and carotenoid content in banana leaves, with EAMP500 showing the highest recovery (chlorophyll: 2.23 ± 0.04 mg/g, carotenoid: 0.18 ± 0.01 mg/g) (p < 0.05). These results suggest that EAMP is a promising, eco-friendly alternative for managing root-knot nematodes in banana cultivation.
Keywords
Millettia pachyloba D
Meloidogyne incognita
Greenhouse conditions
Nematicidal activity
Root-knot nematode
Banana plant protection
International Journal of Horticultural Science and Technology
Volume 13, Issue 3
Summer 2026
Pages 493-506

  • Receive Date 15 December 2024
  • Revise Date 15 May 2025
  • Accept Date 21 May 2025