Effects of Melatonin and Putrescine on the Activity of Antioxidant Enzymes of Nectarine Redgold Cultivar under Cold Storage

Nabizadeh, Esmail

doi:10.22059/ijhst.2025.380328.899

Effects of Melatonin and Putrescine on the Activity of Antioxidant Enzymes of Nectarine Redgold Cultivar under Cold Storage

Document Type : Research paper

Author

Esmail Nabizadeh

Academy membership

10.22059/ijhst.2025.380328.899

Abstract

Under storage conditions, oxidative stress prompts plant cells to enhance their antioxidant system to neutralize reactive oxygen species. Key antioxidant enzymes, including catalase, superoxide dismutase (SOD), and peroxidase, serve as protective mechanisms against oxidative damage, with their activity gradually increasing under cold stress. This study investigated the effects of melatonin and putrescine on enzymatic activity in ‘Redgold’ nectarines using a factorial storage experiment based on a completely randomized design with three replications. The factors included storage duration (control: first d, 10, 20, and 30 d) and varying concentrations of melatonin (control, 50, 100, and 150 μM) and putrescine (control and 2.5 mM). Throughout different storage stages, researchers assessed the activity of antioxidant enzymes (SOD, ascorbate peroxidase [APX], and phenylalanine ammonia-lyase [PAL]), along with malondialdehyde (MDA) levels, electrolyte leakage, and total soluble solids (TSS). The results revealed that the application of melatonin and putrescine significantly enhanced enzymatic activity in ‘Redgold’ nectarines compared to the control. Notably, the combination of 150 μM melatonin and 2.5 mM putrescine yielded the most favorable outcomes.

Keywords

storage

antioxidant enzymes

putrescine

melatonin

soluble solids