Ameneh Tarakemeh; Majid Azizi; Vahid Rowshan; Hassan Salehi; Rosella Spina; François Dupire; Hossein Arouei; Dominique Laurain-Mattar
Abstract
Narcissus spp. are of great importance to pharmaceutical industries because of biosynthesis of Amaryllidaceae alkaloids. N. tazetta with several varieties is a widespread species, native to the Mediterranean region with considerable distribution in Iran. In the present study, calli, roots and bulblets ...
Read More
Narcissus spp. are of great importance to pharmaceutical industries because of biosynthesis of Amaryllidaceae alkaloids. N. tazetta with several varieties is a widespread species, native to the Mediterranean region with considerable distribution in Iran. In the present study, calli, roots and bulblets were regenerated from N. tazetta var. Meskin. In order to report alkaloid profile and quantify galanthamine and lycorine, methanolic extract of different in vitro tissues were subjected to alkaloid purification by SPE (solid phase extraction) method, followed by GC-MS analysis. In general, galanthamine and lycorine were detected in all in vitro tissues, while demethylmaritidine was only identified in bulblets. Bulblet was identified as the best source of main Amaryllidaceae alkaloids regarding the highest quantities of galanthamine and lycorine with 15 and 731 µg/g dry weight (DW) respectively.
Ghasem Esmaeili; Majid Azizi; Hossein Arouiee; Jamil Vaezi
Abstract
The morphological specifications play a substantial role in classification and breeding programs of various plant taxa. In the current study, some macro- and micro-morphological features of Salvia nemorosa, Salvia syriaca, Salvia frigida and Salvia virgata (three accessions), were investigated using ...
Read More
The morphological specifications play a substantial role in classification and breeding programs of various plant taxa. In the current study, some macro- and micro-morphological features of Salvia nemorosa, Salvia syriaca, Salvia frigida and Salvia virgata (three accessions), were investigated using scanning electron microscopy and image analysis program. The completely randomized design (CRD) was used to compare the morphological properties (r=4). Macro-morphological and agronomical measurements showed that Salvia species have great potential for cultivation as medicinal and ornamental plants. The cluster analysis suggested that S. virgata and S. frigida located in the same group; on the other side, S. syriaca and S. nemorosa had more similarity. The micro-morphological results showed that glandular trichomes (GTs) and non-glandular trichomes (NGTs) were widely distributed over the leaf and flower surfaces. The peltate GTs with high-density covered sepal and petal and were observed with low density on the leaves. The comparison with different species showed that the highest size and density of GTs belonged to Salvia virgata. The GTs are large and cover a significant portion of the flower surface (3.85-18.45%). The multicellular GTs were observed in S. nemorosa and S. virgata A1 and A2. The uniseriate and multicellular NGTs covered the adaxial leaf surface in different species. Besides, relative variation in GTs was observed in the three studied S. virgata accessions (15.33- 30.58 trichomes per mm2), which indicates the role of environmental conditions in trichomes formation. A wide range of variation in most of the micro- and macro-morphological traits of Salvia genotypes observed in cultivated condition.
