Effect of In Vitro Calcium Fortification on Regeneration and Microtuberization of Three Selected Irish Potato Varieties

Document Type : Research paper

Authors

1 Department of Horticulture and Food Security, Jomo Kenyatta University of Agriculture and Technology, Juja, Kenya

2 College of Agriculture and Natural Resources, Jomo Kenyatta University of Agriculture and Technology, Juja, Kenya

Abstract

Potato is an important tuber crop that has a unique role in the food security of sub-Saharan Africa. Potato production faces several challenges, including limited sources of clean seed and the occurrence of nutrient deficiencies in plants. The availability of clean and viable seeds is the most prevalent challenge which necessitates the development of new strategies for enhancing production. Understanding the link between in vitro nutrient enhancement and seedling vigor in field conditions can assist in mitigating the adverse effects of these challenges after micro plant transplanting. In vitro experiments were set up to investigate the effects of calcium (Ca) fortification on several potato varieties, i.e. ‘Shangi’, ‘Unica’, and ‘Dutch Robjyn’ regeneration and microtuberization. Cuttings were subcultured onto modified MS media containing five levels of CaCl2 8.8 g L-1, 10.4 g L-1, 12 g L-1, 13.6 L-1, and 15.2 g L-1. Microtubers were initiated on media with 60 g L-1 brown sugar and 6 mg L-1 6-Benzylaminopurine. The experiment was set up in a completely randomized design with three replications. The regeneration capacity of the tested varieties differed significantly, as evidenced by shoot (p=0.0002) and root count (p=<.0001). The response of plants to fortification was variably dependent. The application of 10.4-13.6 g L-1 led to a significant increase (p<0.05) in root count in all three varieties. Furthermore, 13.6 g L-1 CaCl2 led to an increase in root-zone and mid-stem Ca content by 45%, 202%, and 165% in ‘Shangi’, ‘Unica’, and ‘Dutch Robjyn’, respectively, compared to the control. The ‘Dutch Robjyn’ and ‘Shangi’ performed optimally in terms of regeneration and microtuberization, compared to ‘Unica’, under the
effect of 10.4-13.6 g L-1 CaCl2. The results confirmed the optimization of MS regeneration by Ca enhancement as a potential technology for scaling up the production of clean quality seeds. 

Keywords


Aranda-Peres AN, Peres LEP, Higashi EN, Martinelli AP. 2009. Adjustment of mineral elements in the culture medium for the micropropagation of three Vriesea Bromeliads from the Brazilian Atlantic Forest: the importance of calcium. Hortscience 44, 106–112.
Arvin MJ, Habib A, Donnelly DJ. 2005. Effects of calciumconcentrations in medium on microtuberization of potato (Solanum tuberosum L.). Archive of SID. Iranian Journal of Biotechnology 3, 152–156.
Ayieko MW, Tschirley DL, Ayieko David L, MWAT. 2006. Enhancing access and utilization of quality seed for improved food security in Kenya. Working Paper No 272006, 27, 52.
Beals KA. 2019. Potatoes, nutrition, and health. American Journal of Potato Research 96, 102–110. Springer New York LLC. https://doi.org/10.1007/s12230-018-09705-4
Bibikova T, Gilroy S. 2009. Calcium in root hair growth. In AMC Emons and T Ketelaar Eds., Root Hairs. 145– 170. Springer Berlin Heidelberg. https://doi.org/10.1007/978-3-540-79405-9_3
Cybulska J, Pieczywek PM, Zdunek A. 2012. The effect of Ca2+ and cellular structure on apple firmness and acoustic emission. European Food Research and Technology 235, 119–128. https://doi.org/10.1007/s00217-012-1743-6
Evelyne, N. G., Daniel, N. S., Irene, N. O., Ariel, K. B. 2021. Physico-chemical properties of selected Irish potato varieties grown in Kenya. African Journal of Food Science, 15, 10–19. https://doi.org/10.5897/ajfs2020.2025
Gildemacher P, Demo P, Barker I, Kaguongo W, Woldegiorgis G, Wagoire WW, Wakahiu M, Leeuwis C, Struik PC. 2009. A description of seed potato systems in Kenya, Uganda, and Ethiopia. American Journal of Potato Research 86(5), 373–382. https://doi.org/10.1007/s12230-009-9092-0
Gilroy S, Białasek M, Suzuki N, Górecka M, Devireddy AR, Karpiński S, Mittler R. 2016. ROS, calcium, and electric signals: key mediators of rapid systemic signaling in plants. Plant Physiology 171, 1606–1615. https://doi.org/10.1104/pp.16.00434
Habib A, Abdulnour J, Donnelly DJ. 2004. Increased calcium availability improves potato micropropagation and microtuberization. Potato Research 47, 139–149. https://doi.org/10.1007/bf02735980
Hossain MS, Hossain MM, Hossain T, Haque MM, Quamruzzaman M, Sarkar MD. 2019. Varietal response to benzylaminopurine and chlorocholine chloride on in vitro tuberization of potato. Agricultural Research 8, 452–460. https://doi.org/10.1007/s40003-018-0392-9
Hunková J, Gajdošová A, Szabóová M. 2020. Effect of mesos components (MgSO4, CaCl2, KH2PO4) on in vitro shoot growth of blackberry, blueberry, and saskatoon. Plants 9, 1–8. https://doi.org/10.3390/plants9080935
Ibrahim IA, Emara HA, Nower AA, Abodiab AY. 2016. In vitro cultivation of potato plants. International Journal of Current Microbiology and Applied Sciences 5, 858– 868. https://doi.org/10.20546/ijcmas.2016.512.094
Jiang JF, Li JG, Dong YH. 2013. Effect of calcium nutrition on resistance of tomato against bacterial wilt induced by Ralstonia solanacearum. European Journal of Plant Pathology 136, 547–555. https://doi.org/10.1007/s10658-013-0186-7
Joseph N, Anbazhagan M, Srinivasan S. 2015. In vitro growth of potato plant. International Journal of Current Science 17, 29–36. www.currentsciencejournal.info
Kadaja J, Tooming H. 2004. Potato production model based on principle of maximum plant productivity. In Agricultural and Forest Meteorology 127, 1–2. https://doi.org/10.1016/j.agrformet.2004.08.003
Kaguongo W, Maingi G, Barker I, Nganga N. 2013. The value of seed potatoes from four systems in Kenya. American Journal of Potato Research 90, 1–12. https://doi.org/10.1007/s12230-013-9342-z
Karanja AM, Shisanya C, Makokha G. 2014. Analysis of the key challenges facing potato farmers in Oljoro-Orok Division, Kenya. Agricultural Sciences 05, 834–838. https://doi.org/10.4236/as.2014.510088 Kenya NPC. 2019. Potato Variety Catalogue. http://varieties.potato.org.uk/varieties/view/Accord
Kiama J, Mureithi J, Kiiya W. 2006. Improving production of Irish potato (Solanum tuberosum L.) in Kenya: the use of green manure legumes for soil fertility improvement. UoN Digital Repository.
Kozai T, Byoung-Ryong J, Kubota C, Murai Y. 1995. Effects of volume and initial strength of medium on the growth, photosynthesis and ion uptake of potato (Solanum tuberosum L.) plantlet in vitro. Journal of the Japanese Society for Horticultural Science 64, 63–71. https://doi.org/10.2503/jjshs.64.63
Kumar A, Singh UM, Manohar M, Gaur VS. 2015. Calcium transport from source to sink: understanding the mechanism(s) of acquisition, translocation, and accumulation for crop biofortification. Acta Physiologiae Plantarum 37, 1722. https://doi.org/10.1007/s11738-014-1722-6
Leitão N, Dangeville P, Carter R, Charpentier M. 2019. Nuclear calcium signatures are associated with root development. Nature Communications 10. https://doi.org/10.1038/s41467-019-12845-8
Lekamge D, Sasahara T, Yamamoto SI, Hatamoto M, Yamaguchi T, Maki S. 2021. Effect of enhanced CaCl2, MgSO4, and KH2PO4 on improved in vitro growth of potato. Plant Biotechnology 38, 401–408. https://doi.org/10.5511/plantbiotechnology.21.0830a
Machado MP, da Silva ALL, Biasi LA, Deschamps C, Filho JCB, Zanette F. 2014. Influence of calcium content of tissue on hyperhydricity and shoot-tip necrosis of in vitro regenerated shoots of Lavandula angustifolia Mill. Brazilian Archives of Biology and Technology 57, 636– 643. https://doi.org/10.1590/S1516-8913201402165
Marschner H, Marschner P. 1996. Marschner’s Mineral Nutrition of Higher Plants . Edition No. 3. Mbego S. 2019. Kenya can produce between 8M tonnes of potato annually. Standard Digital. https://www.standardmedia.co.ke/article/20013156 52/kenya-s-potential-in-potato production-revealed
Muleta HD, Aga MC. 2019. Role of nitrogen on potato production: reviewing the role of nitrogen on potato production . Journal of Plant Sciences 7, 36–42. https://doi.org/10.11648/j.jps.20190702.11
Muthoni J, Shimelis H, Melis R. 2013. Alleviating potato seed tuber shortage in developing countries: the potential of true potato seeds. Australian Journal of Crop Science 7, 1946–1954.
Muthoni J, Shimelis H, Melis R. 2014. Spread of Bacterial Wilt disease of potatoes in Kenya: Who is to Blame ? International Journal of Horticulture 4, 10–15. https://doi.org/10.5376/ijh.2014.04.0003
Ozgen S, Busse JS, Palta JP. 2011. Influence of root zone calcium on shoot tip necrosis and apical dominance of potato shoot: Simulation of this disorder by ethylene glycol tetraacetic acid and prevention by strontium. HortScience 46, 1358–1362. https://doi.org/10.21273/hortsci.46.10.1358
Reed BM, Wada S, DeNoma J, Niedz RP. 2013. Improving in vitro mineral nutrition for diverse pear germplasm. In Vitro Cellular & Developmental Biology - Plant 49, 343–355. https://doi.org/10.1007/s11627-013-9504-1
Rodrigues VA, Crusciol CAC, Bossolani JW, Moretti LG, Portugal JR, Mundt TT, de Oliveira SL, Garcia A, Calonego JC, Lollato RP. 2021. Magnesium foliar supplementation increases grain yield of soybean and maize by improving photosynthetic carbon metabolism and antioxidant metabolism. Plants 10. https://doi.org/10.3390/plants10040797
Sarkar D, Pandey SK, Chanemougasoundharam A, Sud KC. 2005. The role of calcium nutrition in potato (Solanum tuberosum) microplants in relation to minimal growth over prolonged storage in vitro. Plant Cell, Tissue and Organ Culture 81, 221–227. https://doi.org/10.1007/s11240-004-5213-0
Seabrook JEA, Coleman S, Levy D. 1993. Effect of photoperiod on in vitro tuberization of potato (Solanum tuberosum L.). Plant Cell, Tissue and Organ Culture 34, 43–51. https://doi.org/10.1007/BF00048462
Simon T. 2016. Effects of variety and tuber size on yield and yield component of potato (Solanum tuberosum L.) in Wolaita zone, southern Ethiopia. In Journal of Natural Sciences Research 6. Online. www.iiste.org
Srikum C, Dheeranupattana S, Chotikadachanarong K, Rotarayanont S, Inta A. 2018. The effect of calcium chloride on alleviation of in vitro shoot tip necrosis of Elsholtzia stachyodes. Burapha Science 23, 1770–1778.
Wada S, Niedz RP, DeNoma J, Reed BM. 2013. Mesos components (CaCl2, MgSO4, KH2PO4) are critical for improving pear micropropagation. In Vitro Cellular and Developmental Biology - Plant 49, 356–365. https://doi.org/10.1007/s11627-013-9508-x
Welz B, Sperling M. 2008. Atomic Absorption Spectrometry. Wiley. https://books.google.co.ke/books?id=xSV5hkuI5l8C
White PJ, Broadley MR. 2003. Calcium in plants. Annals of Botany 92, 487–511. https://doi.org/10.1093/aob/mcg164Xing
Y, Zhu ZL, Wang F, Zhang X, Li BY, Liu ZX, Wu XX, Ge SF, Jiang YM. 2021. Role of calcium as a possible regulator of growth and nitrate nitrogen metabolism in apple dwarf rootstock seedlings. Scientia Horticulturae 276. https://doi.org/10.1016/j.scienta.2020.109740
Zakaria M, Hossain M, Mian M, Hossain T, Sultana N. 2008. Effect of nitrogen and potassium on in vitro tuberization of potato. Plant Tissue Culture and Biotechnology 17. https://doi.org/10.3329/ptcb.v17i1.1124
Zhang L, Du L, Poovaiah BW. 2014. Calcium signaling and biotic defense responses in plants. Plant Signaling and Behavior 9, 1–4. https://doi.org/10.4161/15592324.2014.973818
Zhang XP, Ma CX, Sun LR, Hao FS. 2020. Roles and mechanisms of Ca2+ in regulating primary root growth of plants. Plant Signaling and Behavior 15. https://doi.org/10.1080/15592324.2020.1748283