Blue Diode and Red He-Ne Lasers Affect the Growth of Anthocyanin Producing Suspension Cells of Apple

Kazemzadeh-Beneh, Hashem; Mahna, Nasser; Safari, Ebrahim; Motallebi-Azar, Alireza

doi:10.22059/ijhst.2018.234739.196

Document Type : Research paper

Authors

¹ Department of Horticulture Science, Ph.D. Student in Biotechnology & Plant Molecular Genetic, Faculty of Agriculture & Natural Resources, University of Hormozgan, Bandar Abbas, Iran. Department of Horticultural Sciences, Faculty of Agriculture, University of Tabriz, Tabriz, Iran

² Department of Horticultural Sciences, Faculty of Agriculture, University of Tabriz, Tabriz, Iran

³ Department of Atomic & Molecular Physics, Faculty of Physics, University of Tabriz, Tabriz, Iran

https://doi.org/10.22059/ijhst.2018.234739.196

Abstract

Light is an effective factor in cell suspension culture and must be controlled for optimizing cell growth. Growth of anthocyanin producing suspension cells of a red-fleshed genotype of apple (RFA) was assessed in response to blue diode laser (BDL) and red He Ne (RHNL) laser. The suspension cells in L-shaped test tubes were exposed to short-term laser radiation for 20 min in a rotary shaker. The fresh cell weight (FCW), dry cell weight (DCW), cell volume after sedimentation (CVS), cell number (CN) and cell viability (CV) as criteria of cell growth were recorded at 0, 4, 8, 12 days during cell culture. The cell growth was negatively affected in response to BDL and RHNL compared to control and darkness, respectively. The FCW and DCW was enhanced by BDL whereas were not affected by RHNL. Also, only 30.4 mWcm^-2 intensity of BDL could increase CVS in RFA cells. Changes in CN were not displayed by RHNL and BDL. BDL more than RHNL decreased CV. Cell death rates observed due to BDL and RHNL were 40.42% and 33.67%, respectively. All these results showed that these lasers had diverse effects on FRA cell growth, however, these cells were more sensitive to BDL than RHNL especially in higher intensities, presumably because of its damage to cell membrane leading to cell death.

Keywords

References

Anisimov A, Vorob’ev V, Zuikov A. 1997.The influence of laser radiation on the velocity of rotational motion of protoplasm in elodea cells. Laser Physics 5, 1132-1137.

Codron H, Latci A, Pech J.C, Nebie B, Fallot J. 1979. Control of quiescence and viability in auxin-deprived pear cells in batch and continuous culture. Plant Science Letters17, 29-35.

Dobranszki J, Silvada Jaime A.T. 2010. Micropropagation of apple: A review. Biotechnology Advances 28, 462-488.

Green L.M, Miller A.B, Agnew D.A, Greenberg M.L. Li J, Villeneuve J.P, Tibshirani R. 1999. Childhood leukemia and personal monitoring of residential exposures to electric and magnetic fields in Ontario. Cancer Causes Control 10, 233-243.

Hou B.H, Lin C.G. 1996. Rapid optimization of electroporation conditions for soybean and tomato suspension cultured cells. Plant Physiology 111(2), 166.

Kazemzadeh B.H, Mahna N, Safari E, Zaare-Nahandi F, Motallebi-Azar A. 2015. Effects of diode and He-Ne laser on In vitro production of anthocyanin in apple cell suspension culture. International Journal of Horticultural Science and Technology2, 205-212.

Kazemzadeh B.H. 2013. Effect of laser-light radiation in visible spectrum on the production of anthocyanin in suspension cell culture of apple (Mulas sp.). Submitted in partial fulfillment of M.Sc. degree in Horticulture Sciences, University of Tabriz, Tabriz, Iran.

‫ Kazemzadeh B.H, Mahna N, Keramati M, Safari E, Naghshiband hasani R. 2017. Effect of combined he-ne and diode lasers on anthocyanin production and growth in cell suspension cultures of a red-fleshed apple (Malus Sp.). Journal of Plant Process and Function 6 (21), 237-246. (In Persian)

Kurata H, Mochizuki A, Okuda N, Seki M, Furusaki S. 2000. Intermittent light irradiation with second or hour-scale periods controls anthocyanin production by strawberry cells. Enzyme and Microbial Technology 26, 621-629.

Mahna N, Motallebi-Azar A. 2007. In vitro micropropagation of apple (Malus × domestica borkh.) cv. Golden Delicious. Communications in agricultural and applied biological science 72, 235-238.

Mustafa R.N, de Winter W, van Iren F, Verpoorte R. 2011. Initiation, growth and cryopreservation of plant cell suspension cultures. Nature Protocols 6, 715-742.

Park Y.G, Kim M.H, Yang J.K, Chung Y.G, Choi M.S. 2003. Light-susceptibility of camptothecin production from in vitro cultures of Camptotheca acuminata Decne. Biotechnology and Bioprocess Engineering 8, 32-36.

Peloi L.S, Soares R.S, Biondo C.E.G, Souza V.R. Hioka N, Kimura E. 2008. Photodynamic effect of light-emitting diode light on cell growth inhibition induced by methylene blue. Journal of Biosciences 33, 231-237.

Perveen R, Jamil Y, Ashraf M, Ali Q, Iqbal M, Ahmad M.R. 2011. He-Ne laser induced improvement in biochemical, physiological, growth and yield characteristics in sunflower (Helianthus annuus L.). Photochemistry and Photobiolog 87, 1453-1463.

Qiu Z, Yuan M, He Y, Li Y, Zhang L. 2017. Physiological and transcriptome analysis of He-Ne laser pretreated wheat seedlings in response to drought stress. Scientific Reports 7, 2-12.

Ruvinov A.N. 2003. Physical grounds for biological effect of laser irradiation. Journal of Physics 36, 2317-2330.

Salyaev R.K, Dudareva L.V, Lankevich S.V, Makarenko S.P, Sumtsova V.M, Rudikovaskaya E.G. 2007. Effect of low-inensity laser radiation on the chemical composition and structure of lipids in wheat tissue culture. Biological Sciences 412, 87-88.

Stutter G.W, Edney S.L, Newsham G.J. 2005. Photoregulation of anthocyanin production in red leaf lettuce with blue LEDs is affected by timing and leaf age. Limerick Institute of Technology, Limerick, Ireland.

Van Nocker S, Berry G, Najdowski J, Michelutti R, Luffman M,Forsline P. 2012.Genetic diversity of red-fleshed apples (Malus). Euphytica 85, 281-293.

Wi S.G, Chung B.Y, Kim J.H, Beak M.H, Yang D.H, Lee J.W, Kim J.S. 2005. Ultrastuctural changes of cell organelles in Arabidopsis stem after gamma irradiation. Journal of Biology 48(2), 195-200.

Wilson M, Mia N. 1993. Sensitization of Candida albicans to killing by low-power laser light. Journal of Oral Pathology and Medicine 22, 354-357.

Zhong J.J. 2007. Biochemical engineering of the production of plant specific secondary metabolites by cell suspension cultures. State key laboratory bioreactor engineering, East China University of Science and Technology, China.

Blue Diode and Red He-Ne Lasers Affect the Growth of Anthocyanin Producing Suspension Cells of Apple

References

References

Volume 5, Issue 2December 2018Pages 231-239

Volume 5, Issue 2
December 2018
Pages 231-239