University of Tehran International Journal of Horticultural Science and Technology 2322-1461 5 2 2018 12 01 Genetic Variation of Lime (Citrus sp.) Accessions Using Flow Cytometry Technique, Morphological Characteristics and Molecular Markers 199 208 69076 10.22059/ijhst.2018.258553.241 EN Hamed Hassanzadeh Khankahdani The Former Ph.D Student of Fruit Trees Physiology and Breeding, Horticulture Department of Agricultural and Natural Resource Faculty, Hormozgan University and Horticulture Research Department, Agricultural and Natural Resources Research and Education Center, AREEO, Bandar Abbas, Hormozgan, Iran. Somayeh Rastegar Assistant Professor of Horticulture Department of Agricultural and Natural Resource Faculty, Hormozgan University, Iran. Behrouz Golein Associate and Assistant Professor of Citrus and Sub Tropical Fruits Institute, Horticultural Science Research Institute, AREEO, Ramsar, Mazandaran, Iran, Respectively. Morteza Golmohammadi Associate and Assistant Professor of Citrus and Sub Tropical Fruits Institute, Horticultural Science Research Institute, AREEO, Ramsar, Mazandaran, Iran, Respectively. Abdolhossein Aboutalebi Jahromi Horticulture Department, Jahrom Branch, Islamic Azad University, Jahrom, Fars, Iran. Journal Article 2018 05 22 Because of sexual propagation of Mexican lime in southern regions of Iran, there are many lime accessions (lime biotypes) in Mexican lime gardens in which appear some variation in fruit and tree shape. However, most of these accessions are susceptible to witches broom disease of lime (WBDL). Persian lime (<em>C. latifolia</em> Tanaka) is a triploid WBDL-tolerant species. Considerable number of un-described lime biotypes have been cultivated by farmers as Persian lime with no documented evidence related to their origin and genetic background. To unveil systematic modality of the lime accessions, we investigated 18 lime accessions collected from Fars, Hormozgan and Mazandaran provinces (Iran) using flow cytometry (FCM) approach, morphological characteristics, ISSR and SSR markers in 2016. The results of FCM revealed that 14 out of 18 lime accessions were triploid. Triploid accessions showed higher leaf dimension values together with lower stomata and secretory sacs density compared with diploid accessions. Application of SSR markers confirmed the obtained results of ploidy level determination using FCM and morphological analysis. Results of morphological and ISSR markers demonstrated that four out of 18 accessions were different from others and it was in correspondence with the results of FCM. SSR results also grouped the accessions in line of FCM. In conclusion, it has been shown that IFJKh, Cucumber-shaped lime and IFJKMes accessions, are diploid and their cultivation is not recommended in the infected regions to WBDL phytoplasma. https://ijhst.ut.ac.ir/article_69076_34168f45d80294785b51ed893b8ec141.pdf