University of Tehran International Journal of Horticultural Science and Technology 2322-1461 5 1 2018 06 01 Quantitative Assessment of Diosgenin from Different Ecotypes of Iranian Fenugreek (Trigonella foenum-graecum L.) by High-performance Liquid Chromatography 103 109 67915 10.22059/ijhst.2018.254988.230 EN Sahar Aminkar Department of Horticultural Science, Faculty of Agriculture, Tarbiat Modares University, Tehran, Iran Abdolali Shojaeiyan Department of Horticultural Science, Faculty of Agriculture, Tarbiat Modares University, Tehran, Iran. Sajad Rashidi Monfared Department of Plant Biotechnology, Faculty of Agriculture, Tarbiat Modares University, Tehran, Iran. Mahdi Ayyari Department of Horticultural Science, Faculty of Agriculture, Tarbiat Modares University, Tehran, Iran. Journal Article 2018 02 24 In this study, a sensitive, accurate and reproducible high performance liquid chromatographic method was developed for determination of diosgenin from fenugreek (<em>Trigonella foenum-graecum</em>) leaves in 22 ecotypes of Iranian fenugreek. The obtained results showed that the highest concentration of diosgenin with 23.8 mg g<sup>-1</sup>dw was found in Boshruyeh ecotype. While, Mashhad ecotype with 3.51 mg g<sup>-1</sup>dw diosgenin contained the lowest concentration of diosgenin. The method employed in current study confirmed significant variations in the diosgenin concentration in Iranian fenugreek. Therefore, Iranian fenugreek can be considered as a new potential crop and source of diosgenin in the pharmaceutical industry. Dioscin HPLC quantitative determination secondary metabolites steroidal saponin https://ijhst.ut.ac.ir/article_67915_16a49f9d2d7496943c317393fca64850.pdf