University of Tehran International Journal of Horticultural Science and Technology 2322-1461 4 1 2017 06 01 In Vitro Regeneration of Iranian Melon (Cucumis melo L. ‘Samsoori’) Using Antibiotic and Benzyl adenine Micropropagation of Cucumis melo L. ‘Samsoori’ 117 126 65943 10.22059/ijhst.2017.224186.165 EN Davood Naderi Young Researchers Club, Isfahan (Khorasgan) Branch, Islamic Azad University, Isfahan, Iran 0000-0003-1361-8275 Esmaeil Mahmoudi Department of Plant Protection, Faculty of Agriculture and Natural Resources, Isfahan (Khorasgan) Branch, Islamic Azad University, Isfahan, Iran Journal Article 2017 01 03 <em>Cucumis melo</em> L. is one of the most economically important horticultural crops worldwide. However,   low plant regeneration frequency of this plant during genetic transformation is  the  major hurdle for  applying biotechnological approaches. Hence, this study aimed to evaluate the effect of 6-benzyladenine (BA), cefotaxime (CTX), kanamycin (KAN), and indole-3-acetic acid (IAA) on the regeneration of cotyledonary petioles generated from 6-day-old <em>in vitro</em> grown seedlings. Results showed that  application of 1.5 mgl^-1BA plus 250 mgl^-1CTX and 1 mgl^-1 BA with 1000 mgl^-1 CTX formed the most efficient media for plant regeneration. The highest callus production was recorded on medium containing 1 mgl^-1 BA with 250 mgl^-1 CTX and 1.5 mgl^-1 BA with 750 mgl^-1 CTX. Medium containing 500 mgl^-1CTX plus 0.1 mgl^-1IAA  efficiently  induced both root \ and leaf formation. All regenerated plants were died by adding   100 mgl^-1 kanamycin therefore this level considered as threshold level for kanamycin application.. Overall, the results indicated that presence of BA plays an essential role for melon regeneration and cefotaxime can be considered as an auxiliary agent.  https://ijhst.ut.ac.ir/article_65943_55f0b0ad1369cb5c7f8885e341288eac.pdf