University of Tehran International Journal of Horticultural Science and Technology 2322-1461 4 1 2017 06 01 A New Approach for Olive (Arbequina cv.) Micropropagation: Effect of Dikegulac, Light and Carbon Source 79 87 65937 10.22059/ijhst.2018.205988.133 EN Mousa Moradnezhad Biotechnology Department, Faculty of Agricultural Sciences and Natural Resources, Imam Khomeini International University, Qazvin, Iran. Ramin Hosseini Biotechnology Department, Faculty of Agricultural Sciences and Natural Resources, Imam Khomeini International University, Qazvin, Iran. Mohmmad Mehdi Zarrabi Biotechnology Department, Faculty of Agricultural Sciences and Natural Resources, Imam Khomeini International University, Qazvin, Iran. Farzan Ghane Golmohammadi Biotechnology Department, Faculty of Agricultural Sciences and Natural Resources, Imam Khomeini International University, Qazvin, Iran. Journal Article 2016 06 19 Micro-propagation of olive provides the possibility of producing clones of native plants with appropriate attributes and mass reproduction in short period of time, compared to conventional methods. Apical dominance in olive explants limits the growth of lateral branches in vitro. The effect of Woody Plant Medium (WPM), Olive Medium (OM) and Murashig and Skoog (MS) with carbohydrate source (mannitol and sucrose), was investigated for in vitro shoot proliferation of olive. Different concentrations of sodium dikegulac (0.0, 2.5, 5.0, 7.5 and 10 mg L-1) along with 3 mg L-1 zeatin and 0.5 mg L-1 BAP and two light qualities (red and white) were used to overcome apical dominance. Effects of different IBA concentrations on the in vitro root induction were also investigated. More than 74% of explants rooted when IBA was used at 2 mg L-1. OM was the most effective medium, resulting in better and morphologically superior microshoots. Mannitol showed a positive effect on shoot proliferation. Using sodium dikegulac at 5 mg L-1 under white light significantly stimulated axillary bud growth. In the corresponding concentrations of sodium dikegulac, red light had a significant effect on longitudinal growth compared to the white light.

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