Leila Baghazadeh Daryaii; Davood Samsampour; Abdolnabi Bagheri; Majid Askari Seyahooei; Mojdeh Raam
Abstract
Investigating genetic diversity in plant species provides a platform for further insight in plant breeding and conservation. Therefore, in the present study genetic diversity of 13 geographically isolated genotypes of Zhumeria majdea, as a seriously endangered medicine plant growing exclusively in Hormozgan ...
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Investigating genetic diversity in plant species provides a platform for further insight in plant breeding and conservation. Therefore, in the present study genetic diversity of 13 geographically isolated genotypes of Zhumeria majdea, as a seriously endangered medicine plant growing exclusively in Hormozgan province (South of Iran) was studied. To do so, the leaf samples of Z. majdea were collected from the main growing habitats of this spices including Haji Abad, Geno and Bastak regions. The collected leaf samples were subjected to DNA extraction followed by PCR assay, using Inter-Simple Sequence Repeats (ISSR) markers. Twelve markers produced totally 121 polymorphic bands and revealed a clear-cut among and within Z. majdea genotypes. The analysis of molecular variance (AMOVA) showed 86% and 14% variations within and among populations, respectively. Cluster analysis divided genotypes into four main groups. The first and second principle coordinates allocated 28.81% and 15.71% of the variations, respectively. In addition to the innate differences of the individuals, the high intra population variation of Z. majdea, can also be explained by differences in the presence or absence of endophytes and differences in the type and genetic pattern of their endophytes.
Fatemeh Nejadhabibvash; Esmaeil Rezaei Chiyaneh; Alireza Pirzad
Abstract
This study was conducted to investigate the effect of altitudinal gradients on the anatomy of Salvia limbata C.A.Mey. (Lamiaceae). . The S. limbata species were collected from their natural habitats from various regions of West Azerbaijan province in Iran. The thickness of cuticle, collenchymas, ...
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This study was conducted to investigate the effect of altitudinal gradients on the anatomy of Salvia limbata C.A.Mey. (Lamiaceae). . The S. limbata species were collected from their natural habitats from various regions of West Azerbaijan province in Iran. The thickness of cuticle, collenchymas, phloem, xylem and pith of stem and leaf thickness were measured. Moreover, width, length and number of stomata on both adaxial and abaxal epidermal surfaces were analyzed. Statistical analyses were done on a completely randomized design. The results indicated that by increasing altitude leaf thickness increased from 239.1 to 300.1 µm. Number of leaf epidermal cells was also increased in higher altitude on both upper (4.30 to 5.61) and lower (1.05 to 6.55) epidermal surfaces. The results confirmed that more stomata on both adaxial and abaxial epidermis in high altitude samples were associated with presence of narrower and longer stomata on their leaves. Significant differences in the cuticle, collenchyma, xylem, phloem and parenchymatous pith thickness in the stem were observed in all the six studied populations. At higher altitudes thicker cuticle, increased number of collenchyma and wider xylem, phloem and parenchymatous pith were detected. Plants in Mahlamlu region had the thickest studied parameters and Plants in Kabudan Island region had the thinnest tissues.