Mohammad Hadi Ebrahimzadeh; mahboobeh naseri; fatemeh soghra Younesikelaki; iman yusefi javan; Rama Swamy N
Abstract
Silybum marianum (L.) is a medicinally important herb known for producing the liver-protecting compound silymarin, an isomeric mixture of flavonolignans and taxifolin. In this study, a protocol (plant growth regulators in various concentrations and combinations of coconut water) for the initiation of ...
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Silybum marianum (L.) is a medicinally important herb known for producing the liver-protecting compound silymarin, an isomeric mixture of flavonolignans and taxifolin. In this study, a protocol (plant growth regulators in various concentrations and combinations of coconut water) for the initiation of callus and silymarin production using different types of explantes (root, hypocotyls, cotyledonary leaf) of S. marianum L. was established. The free radical scavenging activity of silymarin extracted from callus cultures of these explants was investigated. In comparison to other plant growth regulators and explants studied, root explants cultured on MS medium enriched with 5.0 M NAA + 2.5 M BAP + 10% coconut water produced the most friable callus and had the highest possible level of silymarin and free radical scavenging activity. NAA + BAP + coconut water was found to be the best hormonal combination for callus initiation and mass production. This research will aid in understanding the influence of plant growth regulators and different types of explantes on the production of valuable secondary metabolites in S. marianum L. in vitro cultures.
Sahar Sedaghat; Majid Rahemi; Elham Aslmoshtaghi
Abstract
Hot air treatment has been applied on a wide range of horticultural crops to control postharvest decay and to maintain quality characteristics. To evaluate the effect of hot air treatment on physio-chemical properties of pomegranate arils, an experiment was carried out in a factorial experiment using ...
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Hot air treatment has been applied on a wide range of horticultural crops to control postharvest decay and to maintain quality characteristics. To evaluate the effect of hot air treatment on physio-chemical properties of pomegranate arils, an experiment was carried out in a factorial experiment using a completely randomized design with four replications in 2015. About 200 g of ‘‘Rabab-e-Neyriz’’ pomegranate arils were placed in 350 mg polypropylene boxes with three holes on top for ventilation. Boxes were heated at 35 °C, 40 °C and 45 °C and 80% relative humidity for 30, 60, and 120 min in oven. After being cooled at an ambient temperature, boxes were stored at 5 °C (RH= 70-80%) for 15 days. The scored results of decay assay showed that heated “Rabab” arils at 35 °C till 45 °C decayed lesser than those untreated during storage period. Hot air temperature at 45 °C for 120 min resulted in the highest weight loss at the end of storage period. The highest total soluble solids content was belonged to the arils heated at 45 °C for 30 min. Hot air treatments increased pH and TSS/TA ratio of aril juice. Hot air temperature treatment decreased antioxidant activity, total phenolic compounds and total anthocyanin of arils during storage period, whilst hot air treatment had no significant effect on color values. Generally, the application of mild heat treatments could be considered as a non-contaminant postharvest tool to maintain functional and nutritive properties of arils during postharvest storage.
Ghasem Eghlima; Azizollah Kheiry; Mohsen Sanikhani; Javad Hadian; Mitra Aelaei; Samad Nejad Ebrahimi
Abstract
Licorice (Glycyrrhiza glabra L.) as a strategic and highly valuable medicinal plant in Iran with numerous beneficial pharmaceutical properties contributes substantially to Iranian herbs exports. In the present study, a variation on the phytochemical and antioxidant activity of 25 populations of valuable ...
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Licorice (Glycyrrhiza glabra L.) as a strategic and highly valuable medicinal plant in Iran with numerous beneficial pharmaceutical properties contributes substantially to Iranian herbs exports. In the present study, a variation on the phytochemical and antioxidant activity of 25 populations of valuable and profitable medicinal plant of G. glabra was investigated. The climate variables and soil properties were evaluated in various habitates of G. glabra. Total phenol (Folin–Ciocalteu method), total flavonoid (Aluminum Chloride method), anthocyanin (pH difference method) and antioxidant activity (DPPH method) were studied. Glycyrrhizic acid, glabridin, liquiritigenin and liquiritin content of root were evaluated by liquid chromatography. The content of major components in G. glabra varied in different regions. Outstanding quantitative variability of glycyrrhizic acid, glabridin, liquiritin and liquiritigenin content were observed in roots of licorice from different regions of Iran. Glycyrrhizic acid content was varied from 93.89 (mg/g dry weight) for the Sepidan population to 20.68 (mg/g dry weight) for the Ahar population. The maximum and minimum amounts of glabridin were recorded in Kashmar and Bajgah populations, respectively. The highest levels of liquritin and liquiritigenin were in the population of Kazerun and Yasuj, respectively, and the lowest was observed in Kashmar. Also, the highest antioxidant activity (the lowest IC50) for licorice root was associated with Semirom population. In conclusion, The populations with high amount of each active ingredient in licorice root and those with considerable antioxidant activity can be exploited depending on the purpose of breeding and cultivation.
Hamed Shokri Heydari; Mohammad Ali Askari Sarcheshmeh; Mesbah Babalar; Taha Ranjbar Malidarreh; Ahmad Ahmadi
Abstract
Peach is a highly corrosive fruit with a short shelf life (less than 7 days at room temperature)susceptible to diseases, pathogens and physical damage. The storage or marketable life ofhorticultural crops can be extended by various treatments applied to them after and/or beforeharvesting. Fruits are ...
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Peach is a highly corrosive fruit with a short shelf life (less than 7 days at room temperature)susceptible to diseases, pathogens and physical damage. The storage or marketable life ofhorticultural crops can be extended by various treatments applied to them after and/or beforeharvesting. Fruits are usually treated with a range of materials [(e.g. salicylic acid (SA)] toimprove their appearance or delay deterioration. In the present study, effects of pre-harvesttreatment of SA (1, 2, and 4 mM) and iron sequestrine (Fe) (5 and 10 mg L-1) on antioxidantcapacity, ion leakage, ethylene production, ascorbic acid and carotenoids content of peachfruit (Prunus persica L. Batsch cv. Za'ferani) were examined. The results showed that Fe andSA treatments are effective methods for alleviating ion leakage, weight loss and ethyleneproduction in peach fruit during cold storage. Application of 4.0 mM SA and 10.0 mg L-1 Fewere the most effective treatments to maintain fruits quality parameters. SA and Fe treatmentsmaintained peach fruits quality parameters until 40 days. These results suggest that the SAand Fe treatment are useful materials with potential postharvest application for reducingethylene production, maintain quality, and improve the health benefits of peach fruit byincreasing its antioxidant capacity.
Fatemeh Roshani Bakhsh; Gholam Reza Sharifi-Sirchi; Davood Samsampoor
Abstract
The grape berries due to containing organic acids, sugars, aromatic compounds, phenolic compounds (including anthocyanins, flavanols, flavonols, stilbenes (resveratrol)), tannins, and oil in the pulp, skin, and seed have numerous health benefits for human health. In this study, we investigated genetic ...
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The grape berries due to containing organic acids, sugars, aromatic compounds, phenolic compounds (including anthocyanins, flavanols, flavonols, stilbenes (resveratrol)), tannins, and oil in the pulp, skin, and seed have numerous health benefits for human health. In this study, we investigated genetic and phytochemical characteristics of four famous grapevine cultivars (Shiraz, Sirch, Panje Arous, and Yaghouti) at the maturity stage in 10-20º Brix on a cultivar basis. This research was performed at university of Hormozgan in 2017. The results indicated that Sirch cultivar had the highest total anthocyanin content (2733 mg kg-1 FW), total phenolic content (1666 mg kg-1 FW) and total carotenoid in the skin. High correlation (R2 = 0.951) was observed between cultivars skin’s total anthocyanin and total carotenoid contents. The highest quercetin content (1593 mg kg-1 FW) among the studied cultivars was obtained in Panje Arous cultivar (a pink grape) and Sirch cultivar had the highest delphinidin specific anthocyanin content (65.03 mg kg-1 FW). Among the studied cultivars, Shiraz had the highest total soluble sugar (%19.90) and amount of vinegar (950 ml Kg -1 grapes). Analysis of GC-MS results of vinegar, indicated that the highest rate of ethanol (%98.442) was found in Panje Arous cultivar. DNA sequencing and alignment analysis of F3H, UFGT, DFR, and MybA1 gene sequences showed that there was high homology (>%99) among the studied cultivars, therefore it can be concluded that they are derived from a common ancestor.
Shiva Zaferanchi; Saeid Zehtab Salmasi; Seyed Yahya Salehi Lisar; Mohammad Reza Sarikhani
Abstract
The present study was carried out to investigate biochemical responses of marigold (Calendula officinalis L.) to Bio-organic fertilizers. Effects of co-inoculation of two plant growth-promoting rhizobacteria (PGPR) (Azotobacter sp.145PI and Azospirillum sp.AC49I), humic acid (HA) (10 kg ha−1), ...
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The present study was carried out to investigate biochemical responses of marigold (Calendula officinalis L.) to Bio-organic fertilizers. Effects of co-inoculation of two plant growth-promoting rhizobacteria (PGPR) (Azotobacter sp.145PI and Azospirillum sp.AC49I), humic acid (HA) (10 kg ha−1), vermicompost (VC) (7 T ha−1) and combinations of these treatments were evaluated in two marigold cultivars [Isfahan double flower (DF) and single flower (SF)]. The biosynthesis of leaf protein, soluble and insoluble carbohydrate contents were increased in treated plants, while total free amino acid content was not influenced by treatments. Fertilizers also had positive impact on leaf and flower pigments, total flavonoid content, and total phenolic contents. Maximum amounts of anthocyanins were obtained in the DF cultivar treated by VC+PGPR and VC, which were 11.414 and 11.192 µmol g-1 FW, respectively. The SF cultivar treated by PGPR (36.11 %) and also the same cultivar treated by VC (33.39 %) had the highest antioxidant activities. In general, simultaneous application of fertilizers and also vermicompost were recognized as the best treatment for marigold plants. In conclusion, the findings of the current study confirmed that chemical composition and antioxidant activity of marigold can be positively influenced by Bio-organic fertilizers; therefore they can be used to obtain medicinal plants with improved quality during sustainable agricultural practices.
Mehdi Hadadinejad; Kamran Ghasemi; Amir Ali Mohammadi
Abstract
Blackberry is a highly perishable fruit and its quality decreases very quickly during postharvest period. In this research, two types of container including: oriented poly styrene (OPS), a petroleum-based material, and oriented poly corn starch (OPCS), a bio-based material, was analyzed over 14 days ...
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Blackberry is a highly perishable fruit and its quality decreases very quickly during postharvest period. In this research, two types of container including: oriented poly styrene (OPS), a petroleum-based material, and oriented poly corn starch (OPCS), a bio-based material, was analyzed over 14 days to determine their effects on shelf life of thornless blackberry. Packages were placed in freezer (0˚C), refrigerator (4˚C) and room (25˚C) temperatures in a factorial format based on completely randomized design. Results showed a strong positive correlation between fruit weight and marketability (R2 = 0.726). It was confirm that fruit weight loss and shriveling can be an important reason for marketability reduction in blackberry. Blackberries were survived for 14 days at 0˚C, 8 days at 4˚C and only 3 days at room temperature. A downward trend was observed for pH, TA and TSS during the storage as well as for fruit taste and visual color. Fruits TSS was decreased regardless of the packaging materials and storage. Blackberries that were kept in OPS had significantly higher marketability and lower weight loss in comparison with OPCS that caused a reduction in fruit visual color and marketability. Results indicated that OPCS permeability caused higher fruit weight loss in comparison with other treatments. Blackberries in OPCS container had the highest amount of phenolic components following 14 days at 0˚C, which was significantly more than amount of phenolic components of fruits in OPS container. Compared to OPS, OPCS container did not make any improving in its characteristics to reduce fruit water loss that can be possible by using Nano clay particles.
Hossein Meighani; Mahmood Ghasemnezhad; Davood Bakhshi
Abstract
The pomegranate fruit is a good source of bioactive compounds. The present study has investigated the biochemical and sensory characteristics of the arils of four Iranian commercial pomegranate cultivars namely Malase Shirine Saveh (MSS), Malase Torshe Saveh (MTS), Alak Shirine Saveh (ASS) and Agha Mohammad ...
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The pomegranate fruit is a good source of bioactive compounds. The present study has investigated the biochemical and sensory characteristics of the arils of four Iranian commercial pomegranate cultivars namely Malase Shirine Saveh (MSS), Malase Torshe Saveh (MTS), Alak Shirine Saveh (ASS) and Agha Mohammad Ali (AMA) for several developmental and ripening stages during 45–180 days after fruit set (DAFS). The results showed that the total soluble solids (TSS), individual and total anthocyanin concentrations, and color parameters including chroma and a* values significantly increased, in contrast, the total phenolic concentration and color parameters (L* and hue angle) gradually decreased during developmental stages. Six anthocyanin pigments were found responsible for the red color of pomegranate juice. The quantity and the quality of the anthocyanin pigments were different among the cultivars and the various developmental stages. The predominant anthocyanin pigment at all developmental stages in all cultivars was cyanidin 3,5-diglucosides. The maximum total phenolic concentration was recorded at 45 DAFS for all cultivars. The highest antioxidant activity was recorded at 45 DAFS, and gradually decreased until 135 DAFS. Similar decrease was observed in total phenolic and flavonoid concentrations. Notably, increasing of antioxidant activity at the late-developmental stage was due to induction of the flavonoids and anthocyanins accumulation.
Maryam Bagheri; Mahmood Esna-Ashari; Ahmad Ershadi
Abstract
The objective of this study was to evaluate the storage life and quality of persimmon (Diospyros kaki Thunb.) fruit cv. ‘Karaj’ stored at 0°C for 4 months after postharvest calcium chloride treatment. Fruit weight loss, fruit firmness, total soluble solids, titratable acidity, total phenolic ...
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The objective of this study was to evaluate the storage life and quality of persimmon (Diospyros kaki Thunb.) fruit cv. ‘Karaj’ stored at 0°C for 4 months after postharvest calcium chloride treatment. Fruit weight loss, fruit firmness, total soluble solids, titratable acidity, total phenolic content, soluble tannin content, chilling injury, antioxidant activity, electrolyte leakage, and malondialdehyde content were measured in 0, 15, 30, 45, 60, 75, 90, 105, and 120 days after storage. Peroxidase and catalase enzyme activities were also determined at the end of the storage. Fruits were dipped in the solutions containing 0.5, 1, and 2% (w/v) CaCl2 as well as distilled water only as the control. Results showed that the fruit weight loss and chilling injury significantly decreased by CaCl2 treatments compared with the control. CaCl2 treatments also increased fruit firmness and catalase and antioxidant activities, whereas they reduced MDA content, EL, and peroxidase activity during 4 months of cold storage. The results indicated that CaCl2 application influenced TPC and soluble tannin compared with the control but had no significant effect on TA. The best result was obtained from the 2% CaCl2 treatment in almost all the studied parameters. In general, postharvest CaCl2 treatment of persimmon could decrease chilling symptoms during the cold storage.
