Mohammad Hadi Ebrahimzadeh; Mahboobeh Naseri; Fatemeh Soghra Younesikelaki; Iman Yusefi javan; Rama Swamy N
Abstract
Silybum marianum (L.) is a medicinally important herb known for producing the liver-protecting compound silymarin, an isomeric mixture of flavonolignans and taxifolin. In this study, a protocol (plant growth regulators in various concentrations and combinations of coconut water) for the initiation of ...
Read More
Silybum marianum (L.) is a medicinally important herb known for producing the liver-protecting compound silymarin, an isomeric mixture of flavonolignans and taxifolin. In this study, a protocol (plant growth regulators in various concentrations and combinations of coconut water) for the initiation of callus and silymarin production using different types of explantes (root, hypocotyls, cotyledonary leaf) of S. marianum L. was established. The free radical scavenging activity of silymarin extracted from callus cultures of these explants was investigated. In comparison to other plant growth regulators and explants studied, root explants cultured on MS medium enriched with 5.0 M NAA + 2.5 M BAP + 10% coconut water produced the most friable callus and had the highest possible level of silymarin and free radical scavenging activity. NAA + BAP + coconut water was found to be the best hormonal combination for callus initiation and mass production. This research will aid in understanding the influence of plant growth regulators and different types of explantes on the production of valuable secondary metabolites in S. marianum L. in vitro cultures.
Mina Farsi; Mohammad Reza Fatahi Moghadam; Zabihollah Zamani; Darab Hassani
Abstract
The experiment was carried out during 2015-2016, with the aim of investigating the effects of scion cultivar, rootstock age and hormonal treatments on the success of walnut minigrafting. Analysis of results showed that all studied factors were effective on graft-take and survival of minigrafted walnuts ...
Read More
The experiment was carried out during 2015-2016, with the aim of investigating the effects of scion cultivar, rootstock age and hormonal treatments on the success of walnut minigrafting. Analysis of results showed that all studied factors were effective on graft-take and survival of minigrafted walnuts and other growth traits of the scions. The highest graft-take was observed on one-year-old rootstocks in hormonal treatments of 20 mg/L BA + 12.5 mg/L IBA for ‘Jamal’ and 80 mg/L BA + 50 mg/L IBA for ‘Chandler’. The highest survival rate was observed on one-year-old rootstocks in control treatment and the lowest was on two-year-old rootstocks in hormonal treatment of 80 mg/L BA + 50 mg/L IBA. The highest growth rate of scion belonged to the scions of ‘Jamal’ on one-year-old rootstocks in hormonal treatment of 20 mg/L BA + 12.5 mg/L IBA and the lowest was in scions of ‘Chandler’ on two-year-old rootstocks in hormonal treatments of 20 mg/L BA + 12.5 mg/L IBA and 80 mg/L BA + 50 mg/L IBA. In addition, diameter of the graft union on two-year-old rootstocks was more than on one-year-old ones. Histological studies showed that increase in the diameter of the graft unit on two-year-old rootstocks was due to dead and necrotic tissues at the upper parts of it which acted as a barrier against passage of water and minerals from the rootstock to the scion and reduced the growth of the scion on two-year-old rootstocks.
Mina Farsi; Mohammad Reza Fatahimoghadam; Zabihollah Zamani; Darab Hassani; Ahmad Ahmadi
Abstract
Compared to other techniques of propagation, grafting is the most successful and feasible technique for asexual propagation of walnut plants. There is little information about graft union formation in walnut. Therefore, the objective of this study was to evaluate histological events during graft union ...
Read More
Compared to other techniques of propagation, grafting is the most successful and feasible technique for asexual propagation of walnut plants. There is little information about graft union formation in walnut. Therefore, the objective of this study was to evaluate histological events during graft union formation in Juglans regia L. cv. Chandler scions when minigrafted on the one-year-old seedlings. Cross and longitudinal sections of the graft union were taken for examining different stages of grafting process after 1, 14, 30, 60, 120, 180, 420 and 540 days of minigrafting. One day after grafting, brown necrotic layers were observed at the cut edges. The first callus cells were initiated from cambium layer of rootstock 14 days after grafting but there was weak connection between two parts. New vascular connections between rootstock and scion were observed 30 days after grafting . Vascular connections were increased in central parts of the graft union 60 days after grafting. In mid-summer (180 days after grafting), necrotic layer was almost disappeared in the central longitudinal sections but they were increased especially in the bark, callus and top parts of the graft union due to high temperature and low humidity. In 420 days after grafting, two parts were strongly connected by xylem vessels, but necrotic layer was still remained and observed in some parts. In 540 day after grafting which was coincided with the end of second growing season, two parts were tightly connected to each other and necrotic layer disappeared in most of cross and longitudinal sections.
Hashem Kazemzadeh-Beneh; Nasser Mahna; Ebrahim Safari; Fariborz Zaare-Nahandi; Alireza Motallebiazar
Abstract
Plant cell cultures could be used as an important tool for biochemical production, ranging from natural pigments to pharmaceutical products. Anthocyanin is affected by a variety of factors. Light, an important plant environmental factor, influences the anthocyanin production in vegetative plant tissues. ...
Read More
Plant cell cultures could be used as an important tool for biochemical production, ranging from natural pigments to pharmaceutical products. Anthocyanin is affected by a variety of factors. Light, an important plant environmental factor, influences the anthocyanin production in vegetative plant tissues. Here, we have investigated the influence of the blue laser-diode (BLD) and red laser-He Ne (RLHN) irradiation on the ability of apple cells in producing anthocyanin in suspension culture. Callus was induced from young leaf of apple and cell suspension cultures were subsequently formed from calluses. The normal cell suspension into L-shape tube test exposed to RLHN (6.46, 4.82, 1.54 mWcm-2 and 666.66 μWcm-2) and BLD(67.09, 32.74, 30.4, 13.73 mWcm-2) laser radiation for 20 min. Results showed that theanthocyanin production was enhanced significantly by both RLHN and BRD compared withdarkness and fluorescent light cells (P<0.05). The cell cultures exposed to 67.09 BLD and6.46 RLHN indicated highest total anthocyanin (TA) and total monomeric anthocyanin(TMA) contents, while cell cultures exposed to darkness and fluorescent light revealed lowestTA and TAM content, respectively. In addition, we have found that RLHN nearly increasedTA content as well as BLD. However, the BLD laser was more effective on TAM contentthan RLHN laser. At the minimum intensity, the RLHN laser could enhance TA contentwhereas scarcely affecting TMA content. Nevertheless, the BLD laser improved both TA andTMA contents. These results suggest that TAM production is impressed by quantity morethan quality by laser irradiation.
