Hossein Zarei; Narges Asadi; Hamid Reza Hashemi Petrodi; Seid Javad Mousavizade
Abstract
Galanthus transcaucasicus is still unknown species whose highest distribution is in southern regions of the Caspian Sea in Iran. No precise study has not been done on Galanthus transcaucasicus in the literature so far. This study aimed to collect and introduce the morphological and anatomical characters ...
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Galanthus transcaucasicus is still unknown species whose highest distribution is in southern regions of the Caspian Sea in Iran. No precise study has not been done on Galanthus transcaucasicus in the literature so far. This study aimed to collect and introduce the morphological and anatomical characters of endemic Galanthus transcaucasicus in detail. 50 Galanthus transcaucasicus accessions belonging to 5 populations (Randomly ten plants from each population) were collected from four provinces, including Mazandaran, Gilan, Golestan, and Ardabil of Iran. In total, 12 morphological and ten anatomical parameters were measured. The largest size of flowers was found for K7 from Khalkhal population with (24.22 and 11.43 mm for outer perianth-segments length and width) and (14.26 and 8.68 mm for inner perianth-segments length and width). Anatomical analysis showed that the cells on the lower (abaxial) epidermis were spherical and short, but on the upper (adaxial) epidermis were oblong and strongly elongated. Stomata were occurred on both the adaxial and abaxial surface of leaves (amphistomatous leaves, but on the upper surface, they were located around the central vein. The number of stomata on the upper surface was less (ranging from 0.37 μm to 0.6 μm per mm2). Anatomically, there is a significant difference between populations from different regions. Maximum leaf thickness was found in the Gorgan population (775 μm).
Nayyereh Ghorbani; Esmaeil Chamani; Ali Akbar Shokoohian; Seyyedeh Sanaz Ramezanpour
Abstract
Biochemical diversity may result from geographic and environmental changes. In the present study, 16 ecotypes of yellow flag species were identified and collected through field studies. The identified ecotypes were then grouped into three populations based on their geographical locations. Eight biochemical ...
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Biochemical diversity may result from geographic and environmental changes. In the present study, 16 ecotypes of yellow flag species were identified and collected through field studies. The identified ecotypes were then grouped into three populations based on their geographical locations. Eight biochemical properties, including chlorophyll a, chlorophyll b, total chlorophyll, carotenoids, phenol and flavonoid content, antioxidant capacity, and anthocyanin, were carefully measured by spectrophotometric methods. The biochemical traits were analyzed in a completely randomized design with three replications using SAS software and principal component analysis using SPSS software. The analysis of variance showed that all biochemical traits studied in the ecotypes of the yellow flag showed significant differences (p<0.05; p<0.01). Photosynthetic pigments were most frequently found in ecotype Q, phenol and flavonoids of ecotype K, ecotype O's antioxidant capacity, and ecotype L's anthocyanin content. The studied populations were divided into three main groups using cluster analysis. The results obtained from the correlation table of traits show that the most positive and significant correlations are related to chlorophyll a and total chlorophyll (r = 90%), total chlorophyll and carotenoids (r = 67%), as well as phenol content and flavonoid content (r = 56 %). The results indicate that the studied ecotypes have considerable diversity in terms of biochemical traits.
Leila Baghazadeh Daryaii; Davood Samsampour; Abdolnabi Bagheri; Majid Askari Seyahooei; Mojdeh Raam
Abstract
Investigating genetic diversity in plant species provides a platform for further insight in plant breeding and conservation. Therefore, in the present study genetic diversity of 13 geographically isolated genotypes of Zhumeria majdea, as a seriously endangered medicine plant growing exclusively in Hormozgan ...
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Investigating genetic diversity in plant species provides a platform for further insight in plant breeding and conservation. Therefore, in the present study genetic diversity of 13 geographically isolated genotypes of Zhumeria majdea, as a seriously endangered medicine plant growing exclusively in Hormozgan province (South of Iran) was studied. To do so, the leaf samples of Z. majdea were collected from the main growing habitats of this spices including Haji Abad, Geno and Bastak regions. The collected leaf samples were subjected to DNA extraction followed by PCR assay, using Inter-Simple Sequence Repeats (ISSR) markers. Twelve markers produced totally 121 polymorphic bands and revealed a clear-cut among and within Z. majdea genotypes. The analysis of molecular variance (AMOVA) showed 86% and 14% variations within and among populations, respectively. Cluster analysis divided genotypes into four main groups. The first and second principle coordinates allocated 28.81% and 15.71% of the variations, respectively. In addition to the innate differences of the individuals, the high intra population variation of Z. majdea, can also be explained by differences in the presence or absence of endophytes and differences in the type and genetic pattern of their endophytes.
Akram Ansari; Ali Gharaghani
Abstract
By applying multivariate statistical analysis, this research aimed to estimate the heritability and find relationships between the vegetative and reproductive characteristics of Prunus scoparia and Prunus elaeagnifolia. Twenty genotypes of each species were selected randomly from cultivated populations ...
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By applying multivariate statistical analysis, this research aimed to estimate the heritability and find relationships between the vegetative and reproductive characteristics of Prunus scoparia and Prunus elaeagnifolia. Twenty genotypes of each species were selected randomly from cultivated populations and twenty-two traits including the tree, leaf, flower, nut and kernel attributes were measured. Results showed that there were high levels of genotypic and phenotypic variations among the genotypes of both species. Many of the measurements pertaining to the leaf, flower, nut and kernel, showed very high heritability (H2 >90%) in both species, whilst some traits such as shoot diameter in P. scoparia and kernel moisture in both species had very lower heritability (H2 <50%). Generally, the heritability of measured traits in P. elaeagnifolia were higher than those of P. scoparia, especially foreconomically important traits including yield (H2 = 94 and H2 = 54.61, respectively in P. elaeagnifolia and P. scoparia), nut weight (H2 = 97.83 and H2 = 85.39.61, respectively in P. elaeagnifolia and P. scoparia) and oil percentage (H2 = 75.55 and H2 = 61.43, respectively in P. elaeagnifolia and P. scoparia). Stepwise regression analysis revealed that the most influential factors on yield of P. scoparia, were the fruit set, flower diameter and leaf length, whilst for the P. elaeagnifolia, the yield was mostly determined by fruit set and leaf area. The high genetic diversity and heritability of the studied traits, indicates high genetic potential of this germplasm to be utilized in future breeding programs.
