Mina Taghizadeh; Mousa Solgi
Abstract
An important part of plant in vitro techniques is the sterilization of explants and the maintenance of aseptic conditions. Ideally, sterilizing materials should be effective on a vast range of microorganisms at low density. Nowadays, the use of compounds such as essential oils (EOs) and nanoparticles ...
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An important part of plant in vitro techniques is the sterilization of explants and the maintenance of aseptic conditions. Ideally, sterilizing materials should be effective on a vast range of microorganisms at low density. Nowadays, the use of compounds such as essential oils (EOs) and nanoparticles is applicable in microbiology studies. The main objective of this experiment was to study the substitution probability of silver nanoparticles (SNPs), thymol and carvacrol as novel sterilization agents in the tissue culture of Cynodon dactylon. Explants were sterilized with 70% ethanol for 2 min, and then 30% Clorox for 15 min. Sterilization complementary treatments (SNPs, thymol and carvacrol) were applied at different concentrations (100 and 200 mg l-1) with exposure times of 30, 60 and 120 min. According to the results, infection of bermudagrass explants was controlled successfully by SNPs, thymol and carvacrol. Examination of various concentrations in different exposure times showed that 200 mg L-1 SNPs in combination with 100 mg L-1 thymol in 60 min inhibited microbial growth. Thymol and carvacrol were more effective than SNPs in controlling bacteria and fungi contaminations. Finally, these novel agents could be used as an alternative to common chemical treatments for elimination and control of microbial population explants in in vitro conditions.
Nafiseh Geshnizjany; Asghar Ramezanian; Morteza Khosh-Khui
Abstract
The purpose of this study was to evaluate the effects of Nano-silver (NS), calcium chloride (CaCl2) and their combinations on Gerbera jamesonii ‘Carambole’ cut flowers. The experiment was conducted as a completely randomized design (CRD) with 10 treatments and four replications, with three ...
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The purpose of this study was to evaluate the effects of Nano-silver (NS), calcium chloride (CaCl2) and their combinations on Gerbera jamesonii ‘Carambole’ cut flowers. The experiment was conducted as a completely randomized design (CRD) with 10 treatments and four replications, with three flowers in each replication. Treatments consisted of 5 mg L-1 NS, 1% and 2% CaCl2, 5 mg L-1 NS+ 1% CaCl2, 5 mg L-1 NS + 2% CaCl2, 0.1% and 0.2% CaCl2, 5 mg L-1 NS + 0.1% CaCl2, 5 mg L-1 NS + 0.2% CaCl2 and a control. It was revealed that CaCl2 postharvest spray, NS in a vase solution, as well as their combinations could significantly increase the vase life of gerbera flowers. The longest postharvest life of treated flowers was obtained from 5 mg L-1 NS and 5 mg L-1 NS + 1% CaCl2 treatments. The highest solution uptake was observed in 5 mg L-1 NS, 5 mg L-1 NS + 2% CaCl2 and 5 mg L-1 NS +1% CaCl2 treatments. Furthermore, application of NS and CaCl2 resulted in a reduced loss of relative fresh weight. The application of CaCl2 alone was able to increase all mentioned traits; however, this increase was less than 5 mg L-1 NS, 5 mg L-1 NS + 1% CaCl2 and 5 mg L-1 NS + 2% CaCl2 treatments. Additionally, in vitro cultivation and microscopic counting of microorganisms showed that microorganism growth at the end of flowering stems had been largely restricted by using NS solely or in combination with CaCl2.
