Ali Pourkhaloee; Morteza Khosh-Khui; Rodrigo Barba-Gonzalez
Abstract
In this study, an efficient in vitro method was established for indirect somatic embryogenesis of tuberose from pedicel-derived calluses on MS medium. The effects of 2,4-D, NAA, BAP, and ABA on callus induction, callus growth, embryogenic callus formation, embryo maturation, and plantlet regeneration ...
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In this study, an efficient in vitro method was established for indirect somatic embryogenesis of tuberose from pedicel-derived calluses on MS medium. The effects of 2,4-D, NAA, BAP, and ABA on callus induction, callus growth, embryogenic callus formation, embryo maturation, and plantlet regeneration were evaluated. Combination of 0.5 mg L-1 2,4-D + 0.5 mg L-1 NAA resulted in the highest percentage of callus induction (100%) and callus quality. The highest average of callus growth was achieved at 1 mg L-1 2,4-D + 1 mg L 1 BAP. Embryogenic calluses were induced on the media containing 1 mg L-1 2,4-D after 90 days of subculturing. The highest number of matured somatic embryos per petri dish (81) and callus greening were observed on the medium containing 1 mg L-1 ABA + 45 g L-1 maltose. Further germination of embryos was observed on MS medium containing 10 to 15 ml L-1 goat colostrum and embryos with leaves regenerated after 90 days. Beside induction of osmotic pressure, colostrum is a rich source of organic nitrogen and calcium which play key roles in somatic embryogenesis. The whole plantlets were achieved after 60 days of subculturing on the media containing IBA (0.75 mg L-1) with a survival rate of 88%. In conclusion, the present study provides a suitable somatic embryogenesis system which may serve in micropropagation, genetic transformation, and ploidy manipulation of tuberose.
Elaheh Hashemidehkordi; Seyed Najmmaddin Mortazavi; Pejman Azadi
Abstract
Zantedeschia spp is an important flower in the ornamental plants market. Due to the high demand for this plant in the horticultural industry, it is indispensable to introduce an in vitro protocol for its mass propagation. For this aim, the tubers of calla lily were disinfected in a hot water bath with ...
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Zantedeschia spp is an important flower in the ornamental plants market. Due to the high demand for this plant in the horticultural industry, it is indispensable to introduce an in vitro protocol for its mass propagation. For this aim, the tubers of calla lily were disinfected in a hot water bath with different temperatures (30, 35, 40, 45, and 50 oC) and duration (30 or 35 min). Then explants were cultured on MS medium with different combinations of 6-Benzylaminopurine (BAP) and Kinetin (Kin). Based on the obtained results, the highest disinfection percentage (more than 90%) was obtained at 45 oC for 35 min. Also, the highest proliferation rate (with an average of 15.33 and 14.32 in cv. Orania and cv. Sunclub, respectively) was observed in 2.5 mg L-1 BAP + 1.5 mg L-1 Kin. The highest rooting percentage (100% in both cultivars) and root number per explant (with an average of 4.00 and 3.03 in cv. Orania and Sunclub, respectively) was obtained in 0.5 mg L-1 Indole-3-acetic acid (IAA) + 0.1 mg L-1 Kin, but the highest root length (with an average of 120.0 and 106.6 mm in cv. Orania and Sunclub, respectively) was observed in 1.0 mg L-1 IAA + 0.1 mg L-1 Kin. In MS medium + 2.0 mg L-1 Indole-3-butyric acid (IBA) + 4% Sucrose, the highest number of tubers (with an average of 6.66 and 5.21 in cv. Orania and Sunclub, respectively) was formed. The highest fresh and dry weights (with an average of 948.33 and 851.33 mg in cv. Orania and Sunclub, respectively) of tuber were obtained in 2.0 mg L-1 IBA + 6% sucrose. The rooted and tuberous plants were adapted in the greenhouse successfully.