Ritambhara Bhutani; Susmita Shukla; Nibha Gupta; Shiv Kant Shukla
Abstract
This research aimed at in vitro propagations of bananas originating from India. Banana (Musa spp.) is a nutritious fruit but shows susceptibility to specific diseases. A traditional method of its propagation is through the separation of suckers, although it may culminate in the transmission of nematodes, ...
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This research aimed at in vitro propagations of bananas originating from India. Banana (Musa spp.) is a nutritious fruit but shows susceptibility to specific diseases. A traditional method of its propagation is through the separation of suckers, although it may culminate in the transmission of nematodes, parasitic organisms, and viral diseases. In the past two decades, plant tissue culture techniques have facilitated the production of disease-free plantlets. Tissue culturing bananas can involve different explants, including shoot tips, suckers, leaves, and flower buds. Each responds differently to the presence and absence of light. In the current research, explant cultures were placed in light or dark incubation for identical durations, i.e., one to six months, to monitor their growth and development. The color intensity of the explants changed in response to different photoperiods. Young flower buds, mature flower buds, and suckers developed a higher color intensity when placed in light than in dark conditions. However, the opposite occurred in leaf explants thatgrew optimally in the dark. The results indicated that banana growth occurred productively from sucker explants in different light conditions and variable durations. The results were optimal when employing sucker explants, which exhibited the fastest growth.
Hashem Kazemzadeh-Beneh; Nasser Mahna; Ebrahim Safari; Fariborz Zaare-Nahandi; Alireza Motallebiazar
Abstract
Plant cell cultures could be used as an important tool for biochemical production, ranging from natural pigments to pharmaceutical products. Anthocyanin is affected by a variety of factors. Light, an important plant environmental factor, influences the anthocyanin production in vegetative plant tissues. ...
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Plant cell cultures could be used as an important tool for biochemical production, ranging from natural pigments to pharmaceutical products. Anthocyanin is affected by a variety of factors. Light, an important plant environmental factor, influences the anthocyanin production in vegetative plant tissues. Here, we have investigated the influence of the blue laser-diode (BLD) and red laser-He Ne (RLHN) irradiation on the ability of apple cells in producing anthocyanin in suspension culture. Callus was induced from young leaf of apple and cell suspension cultures were subsequently formed from calluses. The normal cell suspension into L-shape tube test exposed to RLHN (6.46, 4.82, 1.54 mWcm-2 and 666.66 μWcm-2) and BLD(67.09, 32.74, 30.4, 13.73 mWcm-2) laser radiation for 20 min. Results showed that theanthocyanin production was enhanced significantly by both RLHN and BRD compared withdarkness and fluorescent light cells (P<0.05). The cell cultures exposed to 67.09 BLD and6.46 RLHN indicated highest total anthocyanin (TA) and total monomeric anthocyanin(TMA) contents, while cell cultures exposed to darkness and fluorescent light revealed lowestTA and TAM content, respectively. In addition, we have found that RLHN nearly increasedTA content as well as BLD. However, the BLD laser was more effective on TAM contentthan RLHN laser. At the minimum intensity, the RLHN laser could enhance TA contentwhereas scarcely affecting TMA content. Nevertheless, the BLD laser improved both TA andTMA contents. These results suggest that TAM production is impressed by quantity morethan quality by laser irradiation.
