Mehdi Hosseinifarahi; Habib Alah Moazen; Azam Amiri; Mohammad Mahdi Jowkar; Javad Mottaghipisheh
Abstract
Seed priming is an established approach to the aim of accelerating germination and increasing germination percentage, which can be followed by high-quality seedlings and optimal plant growth. Culture media can influence the growth and nutritional status of seedlings at the nursery stage. In order to ...
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Seed priming is an established approach to the aim of accelerating germination and increasing germination percentage, which can be followed by high-quality seedlings and optimal plant growth. Culture media can influence the growth and nutritional status of seedlings at the nursery stage. In order to study the impact of seed priming and culture medium on germination performance and seedling growth, two separate laboratory and greenhouse experiments were planned in which the seeds and seedlings of sweet pepper and eggplant were subjected to various chemicals (i.e. growth regulators and nutrients) in the culture media. The results showed that the chemicals had a significant effect on seed priming by improving seed germination and root length in both plants. While putrescine improved seed germination, the highest germination percentage and root growth of both plants were observed in the salicylic acid treatment. Seed priming by humic acid was more effective than ZnSO4 and KNO3 in encouraging seed germination. The application of peat moss (solely or in combination with other culture media such as cocopeat and perlite) improved the growth parameters and nutritional status of commercially ready seedlings. The highest shoot weight (fresh and dry) in both plants were observed in seedlings grown on peat moss. Apart from improvements in vegetative features, peat moss enhanced the uptake and accumulation of nutrients such as N, P, K, Ca, and Mg, compared to cocopeat and perlite. In general, the results indicated that salicylic acid was the best priming treatment and peat moss was the best culture medium for the commercial production of eggplant and sweet pepper seedlings.AbbreviationsC: Cocopeat, Pe: Perlite, PM: Peat moss, P: Phosphorus, N: Nitrogen, Mg: Magnesium, K: Potassium, Ca: Calcium, SA: Salicylic acid, HA: Humic acid, GP: Germination percentage, Put: Putrescine, KNO3: Potassium nitrate, ZnSO₄: Zinc sulfate, DW: Distilled water, PAs:
Fatemeh Heidarian; Parto Roshandel
Abstract
To evaluate the effect of salicylic acid (SA) on seed germination of black bean plant under saline conditions, seeds were primed with salicylic acid (0, 2, 10, and 20 mM) and germinated under salt stress (0, 50, and 100 mM NaCl). The measured parameters included the percentage and rate of seed germination, ...
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To evaluate the effect of salicylic acid (SA) on seed germination of black bean plant under saline conditions, seeds were primed with salicylic acid (0, 2, 10, and 20 mM) and germinated under salt stress (0, 50, and 100 mM NaCl). The measured parameters included the percentage and rate of seed germination, seedling length and dry weight, malondialdehyde and hydrogen peroxide levels, and activity of catalase, ascorbate peroxidase and guaiacol peroxidase in the seedlings. Results showed the values of germination indices decreased with increasing the level of salt stress. However, SA priming (10 mM) alleviated the harmful effects of salt stress in black bean. SA increased seed germination percentage by 72% and 45% at 50 and 100 mM NaCl respectively, compared to the control condition. Germination rate augmented by 33% (at 50 mM NaCl) and 60% (at 100 mM NaCl) by SA priming compared to the seeds exposed to salt stress alone. Seedlings dry weight (+ 51% at 50 mM and + 34% at 100 mM) and length (+ 57% at 50 mM and + 29% at 100 mM NaCl) were significantly higher by priming with 10 mM salicylic acid, compared to exclusively salt stress-treated seeds. SA priming increased antioxidant enzymes activities and decreased the levels of lipid peroxidation and hydrogen peroxide in salt stressed black bean seedlings. In conclusion, salicylic acid priming (particularly at 10 mM) enhances salt tolerance in black bean via lessening of oxidative stress.
