Houshang Yadegari; Isa Khammari; Baratali Fakheri; Abdorahim Nouri; Taghi Ebadi
Abstract
The fertilization method and plant genotype are two important factors affecting the active ingredients of medicinal plants. Milk thistle (Silybum marianum L.) is one of the most widely distributed medicinal plants worldwide that its seeds have been used widely for treatment of toxic liver damage. In ...
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The fertilization method and plant genotype are two important factors affecting the active ingredients of medicinal plants. Milk thistle (Silybum marianum L.) is one of the most widely distributed medicinal plants worldwide that its seeds have been used widely for treatment of toxic liver damage. In this research, effects of genotype and fertilization type on the quality of milk thistle seeds were investigated. Seeds of two genotypes of milk thistle (Hungarian (A1) and Iranian (A2) genotypes) were cultured and eight fertilization treatments (F1= control treatment (no fertilizer), F2= cow manure, F3= NPK fertilizer, F4= mycorrhizal (Glomus mosseae) inoculation, F5= combination of nitroxin, bio-sulfur and bio-superphosphate, F6= combination of NPK fertilizer and cow manure, F7= combination of arbuscular mycorrhizal fungi inoculation and cow manure, F8= nano-iron chelate) were used. Traits such as seed yield, oil content and the amount of flavonolignans in the seeds were measured. The results showed that the maximum seed yield was obtained in A2*F4 treatment (1376.54 kg h-1) and the lowest was related to A1*F1 (508.99 kg h-1). The average oil content of the samples was about 2.4 mg g-1 and no significant difference was observed. The results of HPLC analysis showed that the mycorrhizal inoculation (F4) in both genotypes led to the achievement of the maximum amount of most important flavonolignans such as silymarin, taxifolin, silydianin, isosilybin B (18.79, 2.80, 5.02 and 4.73 mg g-1, respectively) and an acceptable amount of isosilybin A (2.72 mg g-1), but A1*F4 treatment yielded the best results. In conclusion, use of mycorrhizal inoculation is an effective practice for production of milk thistle seeds with high quality.
sahar aminkar; Abdolali Shojaeiyan; Sajad Rashidi Monfared; Mahdi Ayyari
Abstract
In this study, a sensitive, accurate and reproducible high performance liquid chromatographic method was developed for determination of diosgenin from fenugreek (Trigonella foenum-graecum) leaves in 22 ecotypes of Iranian fenugreek. The obtained results showed that the highest concentration of diosgenin ...
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In this study, a sensitive, accurate and reproducible high performance liquid chromatographic method was developed for determination of diosgenin from fenugreek (Trigonella foenum-graecum) leaves in 22 ecotypes of Iranian fenugreek. The obtained results showed that the highest concentration of diosgenin with 23.8 mg g-1dw was found in Boshruyeh ecotype. While, Mashhad ecotype with 3.51 mg g-1dw diosgenin contained the lowest concentration of diosgenin. The method employed in current study confirmed significant variations in the diosgenin concentration in Iranian fenugreek. Therefore, Iranian fenugreek can be considered as a new potential crop and source of diosgenin in the pharmaceutical industry.
Somaye Amini; Majid Azizi; Mohammad Reza Joharchi; Farid Moradinezhad
Abstract
This experiment was conducted in Ferdowsi University of Mashhad, in 2011 to investigate the allelopathic potential of 68 medicinal and wild plant species belong to 19 plant families grown in Iran. Results showed that among examined plants, stigma and style of Crocus sativus,leaves of Artemisia kopetdaghensis, ...
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This experiment was conducted in Ferdowsi University of Mashhad, in 2011 to investigate the allelopathic potential of 68 medicinal and wild plant species belong to 19 plant families grown in Iran. Results showed that among examined plants, stigma and style of Crocus sativus,leaves of Artemisia kopetdaghensis, Mentha piperita, Zhumeria majdae, Frulago subvelutina, flowers bud of Eugenia caryophyllata, flower of Perovskia abrotanoides, fruits of Melia azedarach and Ruta graveolenhad the strongest inhibitory effects on lettuce seedling growth. Interestingly by using of very low amount of plant samples (10 mg)growth inhibitory effects of these plants were observed by more than 70%. Additionally, the leaf of Atriplex canescens and the flower of Achillea millefolium had the strongest inhibitory effect on radicle growth (more than 75%) compare to the growth of hypocotyl (less than 20%). Here we can suggest that plants with inhibitory effects on growth and development of other plants have the potential to be applied as biological herbicides; this finding can be highlighted as new sustainable herbicides for biological control of weeds.
