Danial Shokouhi; Abdolreza Bagheri; َAlireza Seifi
Abstract
To establish a dynamic and fine suspension culture, four different methods of tomato cell suspension culture were compared. Hypocotyl explants of the tomato cultivar Jina were used for callus induction on Murashige and Skoog (MS) Medium supplemented with three different phytohormone combinations. Then, ...
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To establish a dynamic and fine suspension culture, four different methods of tomato cell suspension culture were compared. Hypocotyl explants of the tomato cultivar Jina were used for callus induction on Murashige and Skoog (MS) Medium supplemented with three different phytohormone combinations. Then, one gram of each type of calli was transferred to 50 mL of liquid MS medium with four combinations of auxins and cytokinins to produce cell suspensions. The growth rate, judged by cell turbidity, cell fresh weight, and cell viability was evaluated. The best suspension culture was obtained by using friable calli formed on MS medium containing 1 mg L-1 NAA and 0.1 mg L-1 kinetin, transferred to the liquid MS supplemented with 2 mg L-1 NAA, 0.2 mg L-1 2, 4-D and 0.2 mg L-1 zeatin.
Atefeh Gholizadegan; َAlireza Seifi
Abstract
Fusarium wilt is one of the most destructive diseases of muskmelon (Cucumis melo L.), which is an economically important disease worldwide causes yield losses in muskmelon growing areas. One of the most effective controlling measures to prevent Fusarium wilt is through host resistance by using resistance ...
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Fusarium wilt is one of the most destructive diseases of muskmelon (Cucumis melo L.), which is an economically important disease worldwide causes yield losses in muskmelon growing areas. One of the most effective controlling measures to prevent Fusarium wilt is through host resistance by using resistance genes. We used developed molecular markers for Fom-2 gene, which confers resistance to race 1 of Fusarium in muskmelon, to screen muskmelon landraces in Khorasane-e-Razavi, Iran. After validation of the markers on a differential set of resistant and susceptible lines, we identified STS312 marker as the polymorphic and easy-to-score marker. Then we used STS312 to genotype plants from five different landraces. Our results suggest that resistance allele of Fom-2 gene is present in two landraces: Eyvankey and Mashhadi. These landraces can be used by muskmelon breeders to enhance resistance to Fusarium wilt in muskmelon.
