Hassan Sarikhani; Hassan Sarikhani-Khorami
Abstract
Cadaman Avimag rootstock is widely used for almonds and peaches. The purpose of this studywas to investigate the effect of light quality on micropropagation, and some morphological andphysiological properties of this rootstock. Single node explants were cultured on WPM and MSmedia containing three levels ...
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Cadaman Avimag rootstock is widely used for almonds and peaches. The purpose of this studywas to investigate the effect of light quality on micropropagation, and some morphological andphysiological properties of this rootstock. Single node explants were cultured on WPM and MSmedia containing three levels of BA (0, 1 and 2 mg L-1) and three levels of NAA (0, 0.05 and0.1 mg L-1). WPM medium containing 2 mg L-1 BA and 0.1 mg L-1 NAA was selected fordifferent light quality treatments including fluorescent, red, and red+blue lights. Results showedthat light quality had a significant effect on shoot proliferation, length and diameter, nodenumber, shoot fresh and dry weights, leaf thickness, stomata number, and chlorophyllconcentration. Red light resulted in higher shoot diameter, but crunchiness and lack of propershoot growth. Moreover, red light produced the lowest amount of chlorophyll in the explants.Leaf thickness and its structural layers under the red light were the lowest among differenttreatments. Combined red+blue light in many factors resembled fluorescent light but inducedmore chlorophyll and a larger stomata size. The quality of light influenced the growth of thisrootstock in vitro, and affected the stem and leaf vegetative traits.
