Leila Baghazadeh Daryaii; Davoud Samsampour; Abdoolnabi Bagheri; Jelveh Sohrabipour
Abstract
Bacterial endophytes grow symbiotically inside plants and improve the growth of their hosts. We evaluated the effects of inoculating macroalgae bacterial endophytes, introduced formerly by our group, Bacillus aquimaris strain OD14, B. megaterium strain AM25, B. zhangzhouensis strain Tv91C, individually ...
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Bacterial endophytes grow symbiotically inside plants and improve the growth of their hosts. We evaluated the effects of inoculating macroalgae bacterial endophytes, introduced formerly by our group, Bacillus aquimaris strain OD14, B. megaterium strain AM25, B. zhangzhouensis strain Tv91C, individually and in combination with each other on the reactive oxygen species (ROS) of scavenging and antioxidant functions, as well as growth characteristics of Mexican lime seedlings under salinity stress. Accordingly, Mexican lime (Citrus aurant-ifolia Swingle.) seedlings were subjected to four salinity levels, i.e. 0, 2000, 4000 and 6000 µs cm-1 in the presence or absence of bacterial endophytes. The results indicated that salinity stress significantly reduced growth, chlorophyll, and carotenoid content of plants lacking endophytes. Combinatory applications with bacterial endophytes significantly improved the above-mentioned parameters under salinity stress. Lipid peroxidation levels were significantly reduced in plants inoculated with bacterial endophytes. Salinity stress significantly increased the activities of ascorbate peroxidase (APX), superoxide dismutase (SOD), glutathione reductase (GR), peroxidase (POD), and catalase (CAT) in salinity conditions. Overall, the inoculation with bacterial endophytes improved salinity tolerance and reduced the accumulation of ROS by increasing their scavenging via an enhanced redox state of glutathione and more effective antioxidant enzyme activities.
Leila Baghazadeh Daryaii; Davood Samsampour; Abdolnabi Bagheri; Majid Askari Seyahooei; Mojdeh Raam
Abstract
Investigating genetic diversity in plant species provides a platform for further insight in plant breeding and conservation. Therefore, in the present study genetic diversity of 13 geographically isolated genotypes of Zhumeria majdea, as a seriously endangered medicine plant growing exclusively in Hormozgan ...
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Investigating genetic diversity in plant species provides a platform for further insight in plant breeding and conservation. Therefore, in the present study genetic diversity of 13 geographically isolated genotypes of Zhumeria majdea, as a seriously endangered medicine plant growing exclusively in Hormozgan province (South of Iran) was studied. To do so, the leaf samples of Z. majdea were collected from the main growing habitats of this spices including Haji Abad, Geno and Bastak regions. The collected leaf samples were subjected to DNA extraction followed by PCR assay, using Inter-Simple Sequence Repeats (ISSR) markers. Twelve markers produced totally 121 polymorphic bands and revealed a clear-cut among and within Z. majdea genotypes. The analysis of molecular variance (AMOVA) showed 86% and 14% variations within and among populations, respectively. Cluster analysis divided genotypes into four main groups. The first and second principle coordinates allocated 28.81% and 15.71% of the variations, respectively. In addition to the innate differences of the individuals, the high intra population variation of Z. majdea, can also be explained by differences in the presence or absence of endophytes and differences in the type and genetic pattern of their endophytes.