Oksana Lastochkina; Darya Garshina; Chulpan Allagulova; Liudmila Pusenkova; Svetlana Garipova; Dilara Maslennikova; Kristina Fedorova; Irina Shpirnaya; Almaz Ibragimov; Igor Koryakov; Azalia Sakhapova; Guzel Yuldasbaeva; Anastasia Dmitrieva; Mohammadhadi Sobhani; Sasan Aliniaeifard
Abstract
Beneficial microorganisms which help plants to grow better especially under stress conditions are known as plant growth-promoting bacteria (PGPB). These biotic agents, especially Bacillus subtilis have well-known role in plant growth promotion and induction of tolerance to stress in plants. They are ...
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Beneficial microorganisms which help plants to grow better especially under stress conditions are known as plant growth-promoting bacteria (PGPB). These biotic agents, especially Bacillus subtilis have well-known role in plant growth promotion and induction of tolerance to stress in plants. They are deemed to act as bio-active and eco-friendly agents to facilitate plant growth under stressful conditions and even to control postharvest decays. Microbial antagonists, including B. subtilis, effectively control postharvest diseases of different fruits, vegetables and flowers, which is manifested in prolonged storage period and shelf/vase life, while preserving qualities and reducing weight losses. In this review paper we highlight the potential benefit of PGPBs especially B. subtilis, as important biotic useful agents to help horticultural plant perform better under stressful conditions and to delay senescence and control the postharvest deterioration through activation of different defense mechanisms. We further elaborate the underlying mechanisms that PGPB used to help plants to cope with stressful conditions. Nevertheless, the mechanisms of PGPB especially B. subtilis action requires further detailed investigations to fully utilize their potentials in horticulture industry.
Hossein Naderi Boldaji; Shirin Dianati Daylami; Sasan Aliniaeifard; Maryam Norouzi
Abstract
Light spectrum is one of the environmental cues that influence plant growth and development. Light is a stimulating factor for induction of somatic embryos during tissue culture practices. To accelerate the direct embryogenesis, six different light spectra including: white (W), red (R), blue (B), green ...
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Light spectrum is one of the environmental cues that influence plant growth and development. Light is a stimulating factor for induction of somatic embryos during tissue culture practices. To accelerate the direct embryogenesis, six different light spectra including: white (W), red (R), blue (B), green (G), red + blue (R+B) and red + far red (R+FR) together with dark condition (D), in combination with thidiazuron (TDZ) in four concentrations (0, 0.5, 1.5 and 3 mg L-1) were used. Inter-simple sequence repeat was used for identification and genetic stability analysis of somatic regenerated plantlets. Intact protocorm explants showed higher potential for direct somatic embryogenesis (DSE) than the other explants. The rate of DSE was highly dependent on the concentration of TDZ and its interaction with light spectra. R and R + FR spectra with 3 mg L-1 TDZ on intact protocorms and R+FR with 3 mg L-1 TDZ were efficient treatments to induce DSE without somaclonal variation. G light spectrum has also significant effects on DSE of protocorm explants. The amplified products showed 26 scorable bands and regenerates were completely identical to the mother plant. In conclusion, this protocol provides way to regenerate plants through embryogenesis, and is a reliable protocol to obtain proper development and genetic stable Phalaenopsis embryos.