Hamed Hassanzadeh Khankahdani; Abdoolnabi Bagheri
Abstract
Date palm (Phoenix dactylifera L.) is a monocot, dioecious and high longevity tropical plant greatly considered due to its high economic importance in Middle East. This investigation was carried out to detect genetic relations among 34 cultivars of Iranian date palm, both males and females, using ISSR ...
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Date palm (Phoenix dactylifera L.) is a monocot, dioecious and high longevity tropical plant greatly considered due to its high economic importance in Middle East. This investigation was carried out to detect genetic relations among 34 cultivars of Iranian date palm, both males and females, using ISSR markers. Accordingly, total DNA was extracted using CTAB method and was subjected to PCR amplification using 12 ISSR primers. Analysis of ISSR data indicated that PIC value varied from 0.3695 to 0.4998 with average of 0.4497. The highest PIC value belonged to (CT)10 G primer (0.4498). Totally, 112 alleles were recognized. According to morphological attributes and ISSR markers, the 34 studied date cultivars divided into eight main groups and different sub-groups, and no significant correlation between grouping made by morphological and molecular markers. Unlike ISSR markers that produced a clear-cut among male and female cultivars, no distinct border was seen among male and female cultivars using morphological attributes. Furthermore, PCA analysis confirmed the grouping made by ISSR markers. In conclusion, ISSR markers have been useful to separate date palm cultivars.
Hamed Hassanzadeh Khankahdani; Somayeh Rastegar; Behrouz Golein; Morteza Golmohammadi; Abdolhossein Aboutalebi Jahromi
Abstract
Because of sexual propagation of Mexican lime in southern regions of Iran, there are many lime accessions (lime biotypes) in Mexican lime gardens in which appear some variation in fruit and tree shape. However, most of these accessions are susceptible to witches broom disease of lime (WBDL). Persian ...
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Because of sexual propagation of Mexican lime in southern regions of Iran, there are many lime accessions (lime biotypes) in Mexican lime gardens in which appear some variation in fruit and tree shape. However, most of these accessions are susceptible to witches broom disease of lime (WBDL). Persian lime (C. latifolia Tanaka) is a triploid WBDL-tolerant species. Considerable number of un-described lime biotypes have been cultivated by farmers as Persian lime with no documented evidence related to their origin and genetic background. To unveil systematic modality of the lime accessions, we investigated 18 lime accessions collected from Fars, Hormozgan and Mazandaran provinces (Iran) using flow cytometry (FCM) approach, morphological characteristics, ISSR and SSR markers in 2016. The results of FCM revealed that 14 out of 18 lime accessions were triploid. Triploid accessions showed higher leaf dimension values together with lower stomata and secretory sacs density compared with diploid accessions. Application of SSR markers confirmed the obtained results of ploidy level determination using FCM and morphological analysis. Results of morphological and ISSR markers demonstrated that four out of 18 accessions were different from others and it was in correspondence with the results of FCM. SSR results also grouped the accessions in line of FCM. In conclusion, it has been shown that IFJKh, Cucumber-shaped lime and IFJKMes accessions, are diploid and their cultivation is not recommended in the infected regions to WBDL phytoplasma.
Abdolhossein Abootalebi Jahromi; Hamed Hassanzadeh Khankahdani
Abstract
Effects of humic acid on some vegetative characteristics and mineral concentrations of Mexican lime were investigated under different salinity concentrations. Four doses of humic acid (0, 1500, 3000 and 4000 mg/kg soil) and four salinity levels (1500, 2500, 3500 and 4500 µmos) were applied on Mexican ...
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Effects of humic acid on some vegetative characteristics and mineral concentrations of Mexican lime were investigated under different salinity concentrations. Four doses of humic acid (0, 1500, 3000 and 4000 mg/kg soil) and four salinity levels (1500, 2500, 3500 and 4500 µmos) were applied on Mexican lime seedlings. Experiment was conducted as a factorial arrangement based on a completely randomized design. The results showed that humic acid had a positive influence on plant height under salinity stress. Shoot number was significantly increased by application of humic acid under salinity stress conditions. In 4500 µmos salinity, application of 4500 mg/kg humic acid caused an increase in shoot fresh and dry weights, root dry weight and shoot potassium concentration. Percentage of sodium was decreased by application of 3000 mg/kg humic acid in the shoot of plants that were exposed to 4500 µmos salinity level. humic acid at 1500 mg/kg caused a reduction in shoot sodium percentage under 3500 µmos salinity level. When compared to the non-treated plants, application of humic acid led to a decline in chloride percentage in Mexican lime seedlings. In conclusion, 3000 and 4000 mg/kg humic acid can be used to reduce the toxic effects of salinity and also to decrease the uptake of toxic elements such as sodium and chloride in Mexican lime seedlings.
