Mina Taghizadeh; Mohammad ali Arab; Mosa Solgi
Abstract
Yield quality and quantity in a hydroponic greenhouse usually relies on optimum crop root health and effective nutrient mobility. This study evaluated the effects of applying arbuscular mycorrhizal fungi and biochar on different growth characteristics of commercial rose cultivars in a hydroponic cultivation ...
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Yield quality and quantity in a hydroponic greenhouse usually relies on optimum crop root health and effective nutrient mobility. This study evaluated the effects of applying arbuscular mycorrhizal fungi and biochar on different growth characteristics of commercial rose cultivars in a hydroponic cultivation system. In this experiment, Rosa hybrida cultivars were ‘Anjelina’ and ‘Dolce Vita’. Treatments included concentrations of 0, 1.5, 3 and 6 g L-1 biochar, and 0 and 7 g L-1 of arbuscular mycorrhizal fungi as organic biofertilizers for the growth of rose plants. Biofertilization at 3 g L-1 biochar and 7 g L-1 arbuscular mycorrhizal fungi optimally increased the flowering shoot diameter, flower diameter, flowering shoot length, and leaf phosphorus content. The ‘Dolce Vita’ cultivar responded more favorably to higher concentrations of arbuscular mycorrhizal fungi and biochar than the ‘Angelina’ cultivar in the hydroponic culture medium. Stem diameter, stem length, and flower diameter in both cultivars improved by higher biochar concentrations. The increased flowering shoot length resulted from root colonization by arbuscular mycorrhizal fungi and increased nutrient uptake, especially phosphorus. A higher nutrient supply increased cell division and shoot growth.
Mina Taghizadeh; Mousa Solgi
Abstract
An important part of plant in vitro techniques is the sterilization of explants and the maintenance of aseptic conditions. Ideally, sterilizing materials should be effective on a vast range of microorganisms at low density. Nowadays, the use of compounds such as essential oils (EOs) and nanoparticles ...
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An important part of plant in vitro techniques is the sterilization of explants and the maintenance of aseptic conditions. Ideally, sterilizing materials should be effective on a vast range of microorganisms at low density. Nowadays, the use of compounds such as essential oils (EOs) and nanoparticles is applicable in microbiology studies. The main objective of this experiment was to study the substitution probability of silver nanoparticles (SNPs), thymol and carvacrol as novel sterilization agents in the tissue culture of Cynodon dactylon. Explants were sterilized with 70% ethanol for 2 min, and then 30% Clorox for 15 min. Sterilization complementary treatments (SNPs, thymol and carvacrol) were applied at different concentrations (100 and 200 mg l-1) with exposure times of 30, 60 and 120 min. According to the results, infection of bermudagrass explants was controlled successfully by SNPs, thymol and carvacrol. Examination of various concentrations in different exposure times showed that 200 mg L-1 SNPs in combination with 100 mg L-1 thymol in 60 min inhibited microbial growth. Thymol and carvacrol were more effective than SNPs in controlling bacteria and fungi contaminations. Finally, these novel agents could be used as an alternative to common chemical treatments for elimination and control of microbial population explants in in vitro conditions.
