Nibras Ihsan Abdul-Jabbar; Azizolah Khandan-Mirkohi; Seyed Alireza salami; Hossein saremi; Samira Shahbazi
Abstract
The biocontrol ability of two strains of Trichoderma fungi on infected Freesia corm by Fusarium disease and eventually growth and performance enhancement of this plant was investigated as laboratory and greenhouse experiment. Laboratory experiment includes five Trichoderma strains as T1, T20, Th4 related ...
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The biocontrol ability of two strains of Trichoderma fungi on infected Freesia corm by Fusarium disease and eventually growth and performance enhancement of this plant was investigated as laboratory and greenhouse experiment. Laboratory experiment includes five Trichoderma strains as T1, T20, Th4 related to T.harzianum species, T2 related to T.artoviride species, and Tvi related to T.virens species. Dual culture test and investigation of the inhibition and colonization rate were carried out with 11 treatments in three replications. Results revealed that strains T1 and T2 have completely covered the pathogen in 96-hours of culture by 70.07% and 68.98% and successfully inhibited the colonization of the disease agent by 84.53% and 80.72%, respectively compared to the other strains. Then, a greenhouse experiment was performed with 8 treatments with suspension of Trichoderma strains separately alone or combined in the presence of Fusarium fungus isolate on the Freesia cultivation based on RCBD design. Results showed that the strains with different levels were able to increase the growth and yield of Freesia plant and control Fusarium disease. In general, T2 strain were more effective in increasing plant growth and height of flower stem of Freesia, while T1 strain specifically improved the diameter of corm. Both T1 and T2 strains demonstrated effectiveness in controlling and reducing the severity of Fusarium disease and enhancing the growth quality of Freesia.Keywords: Biocontrol, Isolates, Ornamental plants, Pathogen, Probiotic, Strains.
Fereshteh Abbasi; Azizolah Khandan-Mirkohi; Ali Haji Ahmad; Mohsen Kafi; Majid Shokrpour
Abstract
This study aimed to optimize an efficient nutrient system to produce cut Lilium flowers while considering the recent severe water crisis and the high cost of chemicals and fertilizers. Lilium bulbs (Lilium OT Hybrid cv. ‘‘Zambesi’’) were grown in aeroponic (centrifugal) and ultrasonic ...
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This study aimed to optimize an efficient nutrient system to produce cut Lilium flowers while considering the recent severe water crisis and the high cost of chemicals and fertilizers. Lilium bulbs (Lilium OT Hybrid cv. ‘‘Zambesi’’) were grown in aeroponic (centrifugal) and ultrasonic systems with variable pulse periods. An ultrasonic system (on/off) time in seconds was considered 10/10, 15/5, and 20/0. An aeroponic system was 3/27, 6/24, and 9/21 in seconds. A modified Hoagland nutrient solution was applied, and plants were grown to the flowering stage. Then, plant morpho-physiological responses were evaluated under different systems and operation times. The growth rate in the vegetative stage and plant height were higher in the aeroponic compared to the ultrasonic system, but the effect of pulses was not significant. Thehighest reproductive growth occurred in 10 and 20% pulses of the aeroponic system. Maximum root length was obtained in 10% of the aeroponic system operation. Maximum but unserious bulb rot was observed in 10% of the aeroponic systems compared to the others. Overall, 10% of the aeroponic system operation was recommendable for Lilium cultivation. Considerably, water consumption was less than half in the ultrasonic system compared to the aeroponic system. Also, the quantum performance of PSII decreased in the time performance by 50%, but it showed better results than the other pulses in the ultrasonic system. The findings reveal that the low water need in the ultrasonicsystem is a promising achievement. Thus, we recommended performing additional experiments with different pulses and even frequencies to achieve better performances in this system.
Shadab Panahi; Azizolah Khandan-Mirkohi; Gail Taylor; Seyed Alireza Salami
Abstract
In this study, we investigated important morphological traits of select populations of Iranian fiber hemp. The analysis of variance showed significant diversity among the populations. Four of the eight genotypes showed a large biomass suitable for the fiber industry. Kerman, Sirjan, Dezful, and Arak ...
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In this study, we investigated important morphological traits of select populations of Iranian fiber hemp. The analysis of variance showed significant diversity among the populations. Four of the eight genotypes showed a large biomass suitable for the fiber industry. Kerman, Sirjan, Dezful, and Arak genotypes had the highest plant height and the largest stem diameter. These populations had late-flowering habits with a short inflorescence length. Our classification revealed that of the four high-fibrous candidates, three were classified in one group, comprising Kerman, Sirjan, and Dezful genotypes. The fourth one, Arak, was placed in the second group. Significantly positive correlations were observed between final plant height, footstalk diameter, and number of days to first-flower emergence. A strong correlation occurred between morphological traits and geographical data pertaining to the genotypes. Our findings indicated that a high elevation and a low average annual temperature are suitable for the growth of medicinal cannabis. Warmer weather made the plants taller with thicker stems and late blooming habits, which are more relevant for use in the fiber industry.
Azizollah Khandan-Mirkohi; Elmira Rabiee; Behrouz Janipour; Ahmad Ahmadi
Abstract
Enrichment of zeolite and sponge as ethylene absorbent with potassium permanganate was theidea of this study to provide an efficient way to scavenge ethylene during storage period ofthree Rosa hybrida cv. ‘Shiraz’, ‘Avalanche’, and ‘After-party’ cut flowers. A preliminaryexperiment ...
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Enrichment of zeolite and sponge as ethylene absorbent with potassium permanganate was theidea of this study to provide an efficient way to scavenge ethylene during storage period ofthree Rosa hybrida cv. ‘Shiraz’, ‘Avalanche’, and ‘After-party’ cut flowers. A preliminaryexperiment revealed that two mL of potassium permanganate solution (1 mM) was enough toenrich two grams of zeolite and two cm3 of sponge. Additionally, one μL L-1 of externalethylene also found to be effective on induction of quality damage to certain cut flowers ofrose cultivars, which were selected for injection in each isolated container for furtherevaluations. The treatments used in this study included control (without absorbent), enrichedzeolite or sponge, enriched zeolite or sponge + ethylene (one μL L-1). The quality of flowers,weight, number of dropped petals, electrical conductivity of vase solution, ethyleneconcentration in the container and petal anthocyanins evaluated through/the end ofexperiment. Results revealed that change or reduction in the quality parameters were minor(more maintenance or stability of quality was achieved) with enriched zeolite and thenenriched sponge compared to the control. Enriched zeolite could even maintain the quality ofcut flowers at the level of control under external ethylene injection superior to enrichedsponge treatment. ‘Avalanche’ and ‘Shiraz’ cultivars figured as the most sensitive andresistant cultivars to the evaluated level of external ethylene concentration, respectively.
Azizolah Khandan-Mirkohi; Mahdieh Khalili Halbi; Seyed Alireza Salami; Hosein Lesani
Abstract
To study effects of salicylic acid (SA) treatments (0, 0.5, 1 and 2 mM) in two forms of seed priming (for 24 h) and spraying on seedling at 4-6 leaf stage an experiment was conducted on periwinkle (Catharanthus roseus Don.) with and without exposure to a mild cold stress of 8 °C (for 32 h). Seeds ...
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To study effects of salicylic acid (SA) treatments (0, 0.5, 1 and 2 mM) in two forms of seed priming (for 24 h) and spraying on seedling at 4-6 leaf stage an experiment was conducted on periwinkle (Catharanthus roseus Don.) with and without exposure to a mild cold stress of 8 °C (for 32 h). Seeds were sown in trays containing peat-based substrate (with 20% sandy loam field soil). Transplants of both groups were treated with mild cold. Seedlings were grown in greenhouse with an average day/night temperature of 25/20± 2°C until their blooming. Seed emergence rate and percentage, activities of catalase (CAT), guaiacol peroxidase (GPX), polyphenol oxidase (PPO), CAT gene expression, height and number of nodes per plant, and days to flowering were evaluated. Results showed that mild cold stress together with salicylic acid at 0.5 mM (as either seed priming or spray on seedling) was the best treatment to accelerate the flowering, and improve growth parameters and antioxidant enzymatic activities. Thus, mild cold stress enhanced the positive effects of SA treatments on cold acclimation of periwinkle (particularly when associated with spraying, which is a simple administration method). Accordingly, it might be recommended for its seedling production and cultivation in temperate climates to prevent late spring frost damages.