Hamed Hassanzadeh Khankahdani; Somayeh Rastegar; Behrouz Golein; Morteza Golmohammadi; Abdolhossein Aboutalebi Jahromi
Abstract
Because of sexual propagation of Mexican lime in southern regions of Iran, there are many lime accessions (lime biotypes) in Mexican lime gardens in which appear some variation in fruit and tree shape. However, most of these accessions are susceptible to witches broom disease of lime (WBDL). Persian ...
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Because of sexual propagation of Mexican lime in southern regions of Iran, there are many lime accessions (lime biotypes) in Mexican lime gardens in which appear some variation in fruit and tree shape. However, most of these accessions are susceptible to witches broom disease of lime (WBDL). Persian lime (C. latifolia Tanaka) is a triploid WBDL-tolerant species. Considerable number of un-described lime biotypes have been cultivated by farmers as Persian lime with no documented evidence related to their origin and genetic background. To unveil systematic modality of the lime accessions, we investigated 18 lime accessions collected from Fars, Hormozgan and Mazandaran provinces (Iran) using flow cytometry (FCM) approach, morphological characteristics, ISSR and SSR markers in 2016. The results of FCM revealed that 14 out of 18 lime accessions were triploid. Triploid accessions showed higher leaf dimension values together with lower stomata and secretory sacs density compared with diploid accessions. Application of SSR markers confirmed the obtained results of ploidy level determination using FCM and morphological analysis. Results of morphological and ISSR markers demonstrated that four out of 18 accessions were different from others and it was in correspondence with the results of FCM. SSR results also grouped the accessions in line of FCM. In conclusion, it has been shown that IFJKh, Cucumber-shaped lime and IFJKMes accessions, are diploid and their cultivation is not recommended in the infected regions to WBDL phytoplasma.
Abdolhossein Aboutalebi Jahromi; Mehdi Hosseini Farahi
Abstract
Marigold has special importance and application in landscape designing. Nowadays, various species and cultivars of this plant are grown in many climates, where different environmental stresses such as freezing, salinity and drought can lead to vegetative disorders. This study was carried out to investigate ...
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Marigold has special importance and application in landscape designing. Nowadays, various species and cultivars of this plant are grown in many climates, where different environmental stresses such as freezing, salinity and drought can lead to vegetative disorders. This study was carried out to investigate the interactions between salinity and ammonium nitrate on seed germination, vegetative growth and sodium and potassium concentrations in French marigold flowers (Tegeta patula). The experiment was conducted as a factorial arrangement based on a completely randomized design with two factors: salinity (0, 2, 4, 6 and 8 mmos cm-1) and ammonium nitrate (0, 15 and 30 g l-1) with four replications. Based on the obtained results, different salinity concentrations were negatively influenced all studied parameters except germination rate. The application of ammonium nitrate recovered the studied parameters to the same level as the control plants. Plant height was decreased by increasing salinity concentrations. The tallest plant was observed in the control treatments. Salinity levels negatively influenced shoot fresh weight. Different levels of ammonium nitrate had significant effects on root dry weight and potassium and sodium contents. Ammonium nitrate led to reductions in root dry weight and potassium content, and an enhancement in sodium content in the shoot. Sodium and potassium levels were increased and root dry weight was decreased by increasing salinity. Enhancement of potassium ions in the marigold following salinity stress can improve its tolerance to salinity stress.
Abdolhossein Abootalebi Jahromi; Hamed Hassanzadeh Khankahdani
Abstract
Effects of humic acid on some vegetative characteristics and mineral concentrations of Mexican lime were investigated under different salinity concentrations. Four doses of humic acid (0, 1500, 3000 and 4000 mg/kg soil) and four salinity levels (1500, 2500, 3500 and 4500 µmos) were applied on Mexican ...
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Effects of humic acid on some vegetative characteristics and mineral concentrations of Mexican lime were investigated under different salinity concentrations. Four doses of humic acid (0, 1500, 3000 and 4000 mg/kg soil) and four salinity levels (1500, 2500, 3500 and 4500 µmos) were applied on Mexican lime seedlings. Experiment was conducted as a factorial arrangement based on a completely randomized design. The results showed that humic acid had a positive influence on plant height under salinity stress. Shoot number was significantly increased by application of humic acid under salinity stress conditions. In 4500 µmos salinity, application of 4500 mg/kg humic acid caused an increase in shoot fresh and dry weights, root dry weight and shoot potassium concentration. Percentage of sodium was decreased by application of 3000 mg/kg humic acid in the shoot of plants that were exposed to 4500 µmos salinity level. humic acid at 1500 mg/kg caused a reduction in shoot sodium percentage under 3500 µmos salinity level. When compared to the non-treated plants, application of humic acid led to a decline in chloride percentage in Mexican lime seedlings. In conclusion, 3000 and 4000 mg/kg humic acid can be used to reduce the toxic effects of salinity and also to decrease the uptake of toxic elements such as sodium and chloride in Mexican lime seedlings.
