Elaheh Hashemidehkordi; Seyed Najmmaddin Mortazavi; Pejman Azadi
Abstract
Zantedeschia spp is an important flower in the ornamental plants market. Due to the high demand for this plant in the horticultural industry, it is indispensable to introduce an in vitro protocol for its mass propagation. For this aim, the tubers of calla lily were disinfected in a hot water bath with ...
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Zantedeschia spp is an important flower in the ornamental plants market. Due to the high demand for this plant in the horticultural industry, it is indispensable to introduce an in vitro protocol for its mass propagation. For this aim, the tubers of calla lily were disinfected in a hot water bath with different temperatures (30, 35, 40, 45, and 50 oC) and duration (30 or 35 min). Then explants were cultured on MS medium with different combinations of 6-Benzylaminopurine (BAP) and Kinetin (Kin). Based on the obtained results, the highest disinfection percentage (more than 90%) was obtained at 45 oC for 35 min. Also, the highest proliferation rate (with an average of 15.33 and 14.32 in cv. Orania and cv. Sunclub, respectively) was observed in 2.5 mg L-1 BAP + 1.5 mg L-1 Kin. The highest rooting percentage (100% in both cultivars) and root number per explant (with an average of 4.00 and 3.03 in cv. Orania and Sunclub, respectively) was obtained in 0.5 mg L-1 Indole-3-acetic acid (IAA) + 0.1 mg L-1 Kin, but the highest root length (with an average of 120.0 and 106.6 mm in cv. Orania and Sunclub, respectively) was observed in 1.0 mg L-1 IAA + 0.1 mg L-1 Kin. In MS medium + 2.0 mg L-1 Indole-3-butyric acid (IBA) + 4% Sucrose, the highest number of tubers (with an average of 6.66 and 5.21 in cv. Orania and Sunclub, respectively) was formed. The highest fresh and dry weights (with an average of 948.33 and 851.33 mg in cv. Orania and Sunclub, respectively) of tuber were obtained in 2.0 mg L-1 IBA + 6% sucrose. The rooted and tuberous plants were adapted in the greenhouse successfully.
Masoumeh Pourghorban; Pejman Azadi; Shahab Khaghani; Abbas Mirzakhani; Mahdi Changizi; Behzad Edrisi
Abstract
In the commercial production of roses, introducing a method to reduce the time of propagation with maximum success is crucial. This study was conducted to investigate the effect of different concentrations (0, 1500, 3000, 4500 mg/L) of Indole-3-butyric acid (IBA) on propagation of three cultivars of ...
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In the commercial production of roses, introducing a method to reduce the time of propagation with maximum success is crucial. This study was conducted to investigate the effect of different concentrations (0, 1500, 3000, 4500 mg/L) of Indole-3-butyric acid (IBA) on propagation of three cultivars of Rosa hybrid L. ('Dolce Vita', 'Samurai' and 'Utopia') by stenting (simultaneous cutting and grafting) method under greenhouse conditions. The cultivars were grafted onto Rosa hybrida L. 'Natal Briar' rootstock. The factorial experiment was performed in a completely randomized design with three replications. The stentings were cultured in coco peat and perlite medium at 1:2 ratio in a greenhouse under mist system. After 60 days, the percentage of grafting, rooting percentage, root number, longest root length and fresh and dry weights of roots and shoot traits (including leaf number, shoot number, and longest shoot length) were determined on the stentings. The highest rate of rooting in Dolce Vita cultivar was obtained at 1500 mg/L IBA and in Samurai and Utopia cultivars at 4500 mg/L IBA. Among the three cultivars of roses, highest percentage of rooting (93.75%), healing percentage (93.75%), root length (12.47 cm), fresh weight (0.84 g) and dry weight (0.07 g) of roots were observed in stentings treated with 4500 mg/L of IBA in Rosa hybrida L. cv. Samurai. Interaction between IBA concentrations and cultivars on chlorophyll (a, b and total) and carotenoid contents were significant in all the three cultivars of roses.
Farzad Nazari; Morteza Khosh-Khui; Pejman Azadi
Abstract
The gerbera market would benefit from an efficient and simple protocol for high rate regeneration for propagation and genetic engineering. With these objectives, this investigation was done on shoot regeneration via direct organogenesis from leafy petiole explants of Gerbera jamesonii ‘Royal Soft ...
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The gerbera market would benefit from an efficient and simple protocol for high rate regeneration for propagation and genetic engineering. With these objectives, this investigation was done on shoot regeneration via direct organogenesis from leafy petiole explants of Gerbera jamesonii ‘Royal Soft Pink’. Murashige and Skoog (1962) (MS) medium was supplemented with 0.1 mg L-1 indole-3-acetic acid (IAA) and additions of various concentrations and combinations of thidiazuron (TDZ: 0, 0.5 and 1 mg L-1) and N6-benzyladenine (BA: 0, 2, 4 and 6 mg L-1). Higher values were recorded for a number of shoots on leafy petiole on the MS medium containing BA in combination with TDZ than on media containing BA or TDZ solely. The highest evaluations for percentage of shoot regeneration (85.43 %) and number of shoots per explant (12.88) was recorded in the medium supplemented with 0.1 mg L-1 IAA and 1.0 mg L-1 TDZ plus 4.0 mg L-1 BA. For rooting of the shoots, MS medium supplemented with three concentrations of a-naphthaleneacetic acid (NAA: 0.5 and 1 mg L-1) together with control (MS only) were tried. The optimal results for rooting of shoots were obtained on MS medium containing 1 mg L-1 NAA. The in vitro raised plantlets were acclimatized and transferred to greenhouse successfully.
Narges Mojtahedi; Parisa Koobaz; Mohammad Fathi; Omid Dabirashrafi; Pejman Azadi; Solmaz Khosravi
Abstract
One of the most effective ways to propagate Lilium is by using tissue culture techniques, preferably through bulblet production. In addition to the surviving percentage of Lilium bulbs after transplantation, the small size of in vitro bulblets compared to commercial bulbs and bulblets‟ dormancy are ...
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One of the most effective ways to propagate Lilium is by using tissue culture techniques, preferably through bulblet production. In addition to the surviving percentage of Lilium bulbs after transplantation, the small size of in vitro bulblets compared to commercial bulbs and bulblets‟ dormancy are the most important constraints to commercializing Lilium micropropagation. Different concentrations of sucrose or sorbitol as carbohydrate sources were investigated as maturation and enlargement factors on in vitro bulblets of Lilium longiflorum cv. „Ceb-dazzle‟. Five concentrations of gibberellic acid and 4°C cold treatment for 6, 7, 8, 9, 10 and 11 weeks in the greenhouse were studied in relation to the dormancy breaking of three in vitro Lilium bulblets cultivars: „Simplon‟, „Navona‟ and „Ceb-dazzle‟, which belong to the Oriental, Asiatic and LA hybrids. The results showed that 3% of sucrose and two weeks of culture significantly increased the bulblet maturation and enlargement of „Ceb-dazzle‟. One mg L-1 gibberellic acid for six weeks, or cold treatment for six to eight weeks, had significant positive effects on the dormancy breaking of in vitro bulblets of Lilium cultivars.
