Hashem Kazemzadeh-Beneh; Nasser Mahna; Ebrahim Safari; Alireza Motallebi-Azar
Abstract
Light is an effective factor in cell suspension culture and must be controlled for optimizing cell growth. Growth of anthocyanin producing suspension cells of a red-fleshed genotype of apple (RFA) was assessed in response to blue diode laser (BDL) and red He Ne (RHNL) laser. The suspension cells in L-shaped ...
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Light is an effective factor in cell suspension culture and must be controlled for optimizing cell growth. Growth of anthocyanin producing suspension cells of a red-fleshed genotype of apple (RFA) was assessed in response to blue diode laser (BDL) and red He Ne (RHNL) laser. The suspension cells in L-shaped test tubes were exposed to short-term laser radiation for 20 min in a rotary shaker. The fresh cell weight (FCW), dry cell weight (DCW), cell volume after sedimentation (CVS), cell number (CN) and cell viability (CV) as criteria of cell growth were recorded at 0, 4, 8, 12 days during cell culture. The cell growth was negatively affected in response to BDL and RHNL compared to control and darkness, respectively. The FCW and DCW was enhanced by BDL whereas were not affected by RHNL. Also, only 30.4 mWcm-2 intensity of BDL could increase CVS in RFA cells. Changes in CN were not displayed by RHNL and BDL. BDL more than RHNL decreased CV. Cell death rates observed due to BDL and RHNL were 40.42% and 33.67%, respectively. All these results showed that these lasers had diverse effects on FRA cell growth, however, these cells were more sensitive to BDL than RHNL especially in higher intensities, presumably because of its damage to cell membrane leading to cell death.
Hashem Kazemzadeh-Beneh; Nasser Mahna; Ebrahim Safari; Fariborz Zaare-Nahandi; Alireza Motallebiazar
Abstract
Plant cell cultures could be used as an important tool for biochemical production, ranging from natural pigments to pharmaceutical products. Anthocyanin is affected by a variety of factors. Light, an important plant environmental factor, influences the anthocyanin production in vegetative plant tissues. ...
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Plant cell cultures could be used as an important tool for biochemical production, ranging from natural pigments to pharmaceutical products. Anthocyanin is affected by a variety of factors. Light, an important plant environmental factor, influences the anthocyanin production in vegetative plant tissues. Here, we have investigated the influence of the blue laser-diode (BLD) and red laser-He Ne (RLHN) irradiation on the ability of apple cells in producing anthocyanin in suspension culture. Callus was induced from young leaf of apple and cell suspension cultures were subsequently formed from calluses. The normal cell suspension into L-shape tube test exposed to RLHN (6.46, 4.82, 1.54 mWcm-2 and 666.66 μWcm-2) and BLD(67.09, 32.74, 30.4, 13.73 mWcm-2) laser radiation for 20 min. Results showed that theanthocyanin production was enhanced significantly by both RLHN and BRD compared withdarkness and fluorescent light cells (P<0.05). The cell cultures exposed to 67.09 BLD and6.46 RLHN indicated highest total anthocyanin (TA) and total monomeric anthocyanin(TMA) contents, while cell cultures exposed to darkness and fluorescent light revealed lowestTA and TAM content, respectively. In addition, we have found that RLHN nearly increasedTA content as well as BLD. However, the BLD laser was more effective on TAM contentthan RLHN laser. At the minimum intensity, the RLHN laser could enhance TA contentwhereas scarcely affecting TMA content. Nevertheless, the BLD laser improved both TA andTMA contents. These results suggest that TAM production is impressed by quantity morethan quality by laser irradiation.